Kinetics of glucose mutarotation assessed by an equal-amplitude paired polarized heterodyne polarimeter.
Kinetics of glucose mutarotation assessed by an equal-amplitude paired polarized heterodyne polarimeter.
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通过等幅配对偏振外差旋光计评估葡萄糖变旋动力学。
DOI：
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发表时间：
2010
影响因子：
2.9
通讯作者：
C. Chou
中科院分区：
文献类型：
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作者：
Chu;Chih;Chuanwei Chen;Li;C. Chou
为了精确测量三蒸水中D-葡萄糖的变旋速率常数，建立了双频等幅配对偏振外差旋光计（DEPHP）。 DEPHP 基于平衡检测器检测方案，用于测量非平衡状态下 α-D-葡萄糖和 β-D-葡萄糖之间转化过程中 D-葡萄糖/水溶液的旋光角。 DEPHP可以进行散粒噪声限制的检测，从而可以高灵敏度地测量总旋光角以及α-D-葡萄糖和β-D-葡萄糖转化的变旋速率常数。本实验中，旋光角测量的灵敏度为8.3×10(-5)deg/cm，而D-葡萄糖的总变旋速率常数(k)、正向变旋速率常数(k(1))和反向变旋速率常数(k(2))为k=7.67×10(-5)s(-1)，k(1)=2.76×10(-5)s(-1)，在三蒸馏水中，k(2) = 4.91 x 10(-5) s(-1)。此外，使用DEPHP，我们可以在632.8 nm处测量水中α-D-葡萄糖和β-D-葡萄糖的比旋转角。它们分别为 105 度和 12 度。最后还讨论了DEPHP系统的检测灵敏度。
A dual-frequency equal-amplitude paired polarization heterodyne polarimeter (DEPHP) was set up in order to precisely measure the mutarotation rate constants of D-glucose in tridistilled water. The DEPHP is based on a balanced detector detection scheme for measurement of the optical rotation angle of D-glucose/water solution during the conversion process between alpha-D-glucose and beta-D-glucose while in a nonequilibrium state. The DEPHP can perform shot-noise-limited detection, so that the total optical rotation angle together with the mutarotation rate constants of alpha-D-glucose and beta-D-glucose conversion can be measured with high sensitivity. In this experiment, the sensitivity of the optical rotation angle measurement was 8.3 x 10(-5) deg/cm, while the total (k), forward (k(1)) and reverse (k(2)) mutarotation rate constants of D-glucose were found to be k = 7.67 x 10(-5) s(-1), k(1) = 2.76 x 10(-5) s(-1), and k(2) = 4.91 x 10(-5) s(-1), respectively, in tridistilled water. Moreover, using the DEPHP, we can measure the specific rotation angles of alpha-D-glucose and beta-D-glucose in water at 632.8 nm. They were 105 degrees and 12 degrees, respectively. Finally, the detection sensitivity of the DEPHP system is also discussed.