Recombinant fish parvalbumins: Candidates for diagnosis and treatment of fish allergy
Recombinant fish parvalbumins: Candidates for diagnosis and treatment of fish allergy
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重组鱼小白蛋白：诊断和治疗鱼过敏的候选药物
DOI：
10.1034/j.1398-9995.57.s72.21.x
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发表时间：
2002
期刊：
影响因子：
12.4
通讯作者：
S. Spitzauer
中科院分区：
文献类型：
--
作者：
Ines Swoboda;A. Bugajska;Rudolph Valenta;S. Spitzauer
鱼和鱼产品是导致IgE介导的食物过敏的最重要原因之一。在致敏个体中，接触和食用鱼类可能导致严重的健康问题，从麻风和皮炎到血管水肿、腹泻、哮喘，最糟糕的情况是全身过敏反应和死亡。小蛋白是一种存在于脊椎动物肌肉中的小钙结合蛋白，被确定为鱼的主要过敏原。通过对鲤鱼肌肉表达文库进行IgE免疫筛选，分离和鉴定了鲤鱼小白蛋白的基因克隆。这些克隆将为生产重组鲤鱼小白蛋白奠定基础，这是一种在 体外和 体内诊断鱼类过敏的有用工具。
Fish and fish products represent one of the most important causes of IgE‐mediated food hypersensitivity. In sensitized individuals contact with and consumption of fish can lead to severe health problems, ranging from urticaria and dermatitis to angiedema, diarrhoea, asthma and, at worst, systemic anaphylactic reactions and death. Parvalbumin, a small calcium‐binding protein present in the muscles of vertebrates, was identified as the major fish allergen. We describe the isolation and characterization of cDNA clones coding for carp parvalbumin by IgE immunoscreening of a carp muscle expression library. These clones will be the basis for the production of recombinant carp parvalbumin, a useful tool for in vitro and in vivo diagnosis of fish allergy.