Assessing drug-cell and drug-tissue interactions through spectral FRET imaging
Assessing drug-cell and drug-tissue interactions through spectral FRET imaging
复制标题
DOI：
10.1117/12.2583249
复制
发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
S. Leavesley;N. Annamdevula;A. Geurts;D. Pleshinger;T. Rich
中科院分区：
文献类型：
--
作者：
S. Leavesley;N. Annamdevula;A. Geurts;D. Pleshinger;T. Rich
结构的复杂性和异质性可能在病理生理学和治疗效果中发挥关键作用，从亚细胞(Nm)到整个器官(Cm)。在这里，我们报告了利用多维光谱FRET成像方法来可视化药物治疗对组织的影响的努力。我们已经建立了一个表达cAMP FRET报告的转基因大鼠系，用于可视化组织中第二信使信号对治疗和病理条件的反应。当与血管和肺制剂一起使用时，转基因FRET组织显示出在单细胞中激发激动剂诱导的cAMP反应的能力。
Structural complexity and heterogeneity may play critical roles in pathophysiology and therapeutic effect, at length scales ranging from subcellular (nm) to whole organ (cm). Here, we report on efforts to utilize a multidimensional spectral FRET imaging approach for visualization of the effects of pharmacologic treatments on tissues. We have developed a transgenic rat line expressing a cAMP FRET reporter for visualization of second messenger signaling in tissues in response to treatment and pathological conditions. When utilized with vascular and lung preparations, transgenic FRET tissues displayed the ability to elicit agonist-induced cAMP responses in single cells.