Histones and basic polypeptides activate Ca2+/cation influx in various cell types
Histones and basic polypeptides activate Ca2+/cation influx in various cell types
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DOI：
10.1042/bj3310623
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发表时间：
1998-04-15
影响因子：
4.1
通讯作者：
Benedetti, A
中科院分区：
文献类型：
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作者：
Gamberucci, A;Fulceri, R;Benedetti, A
在Ehrlich腹水细胞悬液中加入组蛋白H_2A(1-10mU·g/ml)可促进：(1)钙离子内流，但未见明显的细胞内钙动员；(2)无钙介质中质膜去极化和Na+内流；(3)其他阳离子如Ba2+、Mn2+、胆碱(+)和N-甲基-D-葡萄糖胺(+)内流，而丙二醛(+)、乙二溴胺和台盼蓝无此作用。H_2A诱导的钙内流和细胞去极化是：(I)被La3+和Gd3+阻断，但不被受体激活的钙内流途径/通道的各种抑制剂阻断；(Ii)被各种碱性多肽模拟，具有M-r>；4000；(Iii)被聚谷氨酸或肝素等多阴离子阻止或逆转；(Iv)存在于其他类型的细胞，如Jurkat，PC12和Friend红白血病细胞，但几乎不存在大鼠肝细胞和胸腺细胞。我们得出结论，阳离子蛋白/多肽通过与细胞表面以细胞特有的方式相互作用，可以激活这些细胞中可能的非选择性钙通道和膜去极化。
Histone H2A (1-10 mu g/ml) added to Ehrlich ascite cell suspensions promoted: (i) Ca2+ influx, but no apparent intracellular Ca2+ mobilization; (ii) plasma-membrane depolarization and Na+ influx in Ca2+-free medium, which were recovered by Ca2+ readmission; (iii) influx of other cations such as Ba2+, Mn2+, choline(+) and N-methyl-D-glucamine(+), but not of propidium(+), ethidium bromide and Trypan Blue. H2A-induced Ca2+ influx and cell depolarization were: (i) blocked by La3+ and Gd3+, but not by various inhibitors of receptor-activated Ca2+-influx pathways/channels; (ii) mimicked by various basic polypeptides, with M-r > 4000; (iii) prevented or reversed by polyanions such as polyglutamate or heparin; (iv) present in other cell types, such as Jurkat, PC12 and Friend erythroleukaemia cells, but virtually absent from rat hepatocytes and thymocytes. We conclude that cationic proteins/ polypeptides, by interacting in a cell-specific manner with the cell surface, can activate in those cells putative non-selective Ca2+ channels and membrane depolarization.