Cellular regulation of extension and retraction of pseudopod-like blebs produced by nanosecond pulsed electric field (nsPEF).
Cellular regulation of extension and retraction of pseudopod-like blebs produced by nanosecond pulsed electric field (nsPEF).
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DOI：
10.1007/s12013-014-9831-9
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发表时间：
2014-07
影响因子：
2.6
通讯作者：
Pakhomov, Andrei G.
中科院分区：
文献类型：
--
作者：
Rassokhin, Mikhail A.;Pakhomov, Andrei G.
关键词：
Recently we described a new phenomenon of anodotropic pseudopod-like blebbing in U937 cells exposed to nanosecond pulsed electric field (nsPEF). In Ca2+-free buffer such exposure initiates formation of pseudopod-like blebs (PLBs), protrusive cylindrical cell extensions that are distinct from apoptotic and necrotic blebs. PLBs nucleate predominantly on anode-facing cell pole and extend towards anode during nsPEF exposure. Bleb extension depends on actin polymerization and availability of actin monomers. Inhibition of intracellular Ca2+, cell contractility and RhoA produced no effect on PLB initiation. Meanwhile, inhibition of WASP by wiskostatin causes dose-dependent suppression of PLB growth. Soon after the end of nsPEF exposure PLBs lose directionality of growth and then retract. Microtubule toxins nocodazole and paclitaxel did not show immediate effect on PLBs; however, nocodazole increased mobility of intracellular components during PLB extension and retraction. Retraction of PLBs is produced by myosin activation and corresponding increase in PLB cortex contractility. Inhibition of myosin by blebbistatin reduces retraction while inhibition of RhoA-ROCK pathway by Y-27632 completely prevents retraction. Contraction of PLBs can produce cell translocation resembling active cell movement. Overall, the formation, properties, and lifecycle of PLBs share common features with protrusions associated with amoeboid cell migration. PLB lifecycle may be controlled through activation of WASP by its upstream effectors such as Cdc42 and PIP2, and main ROCK activator - RhoA. Parallels between pseudopod-like blebbing and motility blebbing may provide new insights into their underlying mechanisms.
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DOI：
10.1038/nrm2867
发表时间：
2010-04
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
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DOI：
10.1083/jcb.200802081
发表时间：
2008-06-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fackler OT;Grosse R
通讯作者：
Grosse R
DOI：
10.1073/pnas.1207968109
发表时间：
2012-09-04
影响因子：
11.1
作者：
Bergert, Martin;Chandradoss, Stanley D.;Paluch, Ewa
通讯作者：
Paluch, Ewa
DOI：
10.1042/bss0700277
发表时间：
2003-01-01
期刊：
PROTEASES AND THE REGULATION OF BIOLOGICAL PROCESSES
影响因子：
--
作者：
Friedl, P;Wolf, K
通讯作者：
Wolf, K
影响因子：
4.8
作者：
Amano, M;Ito, M;Kaibuchi, K
通讯作者：
Kaibuchi, K