Changes in Bacillus Spore Small Molecules, rRNA, Germination, and Outgrowth after Extended Sublethal Exposure to Various Temperatures: Evidence that Protein Synthesis Is Not Essential for Spore Germination
Changes in Bacillus Spore Small Molecules, rRNA, Germination, and Outgrowth after Extended Sublethal Exposure to Various Temperatures: Evidence that Protein Synthesis Is Not Essential for Spore Germination
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DOI：
10.1128/jb.00583-16
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发表时间：
2016-12-01
影响因子：
3.2
通讯作者：
Setlow, Peter
中科院分区：
文献类型：
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作者：
Korza, George;Setlow, Barbara;Setlow, Peter
据先前报道，枯草芽孢杆菌休眠孢子在50℃的长时间孵育过程中，rrna降解率为95% （E. Segev， Y. Smith, and S. Ben-Yehuda, Cell 148: 139 -114, 2012, doi: http://dx）。doi。org/10.1016/j。细胞。2011.11.059)，巨芽孢杆菌孢子也是如此。这两个物种的孢子在75 ~ 80℃下孵育大约20小时，也导致了全部或绝大部分23S和16S rRNA的降解，尽管这种rRNA的降解比在这些温度下纯化的rRNA的非酶水解慢。这种rRNA在高温下的降解几乎完全产生寡核苷酸和最低水平的单核苷酸。RNase Y被认为参与枯草芽孢杆菌孢子在50℃孵育过程中rRNA的水解，但在80℃时不参与枯草芽孢杆菌孢子rRNA的分解。在70℃下孵育20小时，休眠孢子中已经最低的ATP水平也降低了10- 30倍
rRNAs of dormant spores of Bacillus subtilis were> 95% degraded during extended incubation at 50 degrees C, as reported previously (E. Segev, Y. Smith, and S. Ben-Yehuda, Cell 148: 139 -114, 2012, doi: http://dx. doi. org/10.1016/j. cell. 2011.11.059), and this was also true of spores of Bacillus megaterium. Incubation of spores of these two species for similar to 20 h at 75 to 80 degrees C also resulted in the degradation of all or the great majority of the 23S and 16S rRNAs, although this rRNA degradation was slower than nonenzymatic hydrolysis of purified rRNAs at these temperatures. This rRNA degradation at high temperature generated almost exclusively oligonucleotides with minimal levels of mononucleotides. RNase Y, suggested to be involved in rRNA hydrolysis during B. subtilis spore incubation at 50 degrees C, did not play a role in B. subtilis spore rRNA breakdown at 80 degrees C. Twenty hours of incubation of Bacillus spores at 70 degrees C also decreased the already minimal levels of ATP in dormant spores 10-to 30-fold, to