Molecular characterization of multidrug-resistant Shigella spp. of food origin
Molecular characterization of multidrug-resistant Shigella spp. of food origin
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DOI：
10.1016/j.ijfoodmicro.2014.11.013
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发表时间：
2015-02-02
影响因子：
5.4
通讯作者：
Shimamoto, Tadashi
中科院分区：
文献类型：
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作者：
Ahmed, Ashraf M.;Shimamoto, Tadashi
志贺菌是食源性志贺氏菌病（一种急性肠道感染）的病原体。多重耐药志贺菌临床分离株的出现对临床志贺菌病的治疗提出了越来越大的挑战。世界范围内的几项研究已经表征了临床人源志贺菌分离株抗生素耐药性的分子基础，然而，迄今为止，尚未报道志贺菌属的此类表征。食物来源。在这项研究中，我们的特点是在志贺菌属多药耐药的遗传基础。从埃及不同的街头小贩、屠夫、零售市场和屠宰场收集的1600份食品样品（800份肉制品和800份乳制品）中分离出。27株志贺菌中有24株（88.9%）对至少3种抗菌药物表现出多重耐药表型。福氏志贺菌（66.7%）、宋内氏志贺菌（18.5%）、痢疾杆菌（3.7%）。耐药率最高的是链霉素（100.0%），其次是卡那霉素（95.8%）、萘啶酸（95.8%）、四环素（95.8%）、大观霉素（93.6%）、氨苄西林（87.5%）和磺胺甲恶唑/甲氧苄啶（87.5%）。PCR和DNA测序用于筛选和表征整合子和抗生素耐药基因。结果表明，11.1%和74.1%的分离株为1类和2类整合子阳性。77.8%的分离株检出β-内酰胺酶编码基因，44.4%的分离株检出质粒介导的喹诺酮类耐药基因。这些数据提供了有用的信息，以更好地了解抗生素耐药的志贺菌属的分子基础。据我们所知，这是第一份志贺菌耐药性分子特征的报告。与食物隔绝。(C)2014爱思唯尔有限公司版权所有。
Shigella spp. are the causative agents of food-borne shigellosis, an acute enteric infection. The emergence of multidrug-resistant clinical isolates of Shigella presents an increasing challenge for clinicians in the treatment of shigellosis. Several studies worldwide have characterized the molecular basis of antibiotic resistance in clinical Shigella isolates of human origin, however, to date, no such characterization has been reported for Shigella spp. of food origin. In this study, we characterized the genetic basis of multidrug resistance in Shigella spp. isolated from 1600 food samples (800 meat products and 800 dairy products) collected from different street venders, butchers, retail markets, and slaughterhouses in Egypt. Twenty-four out of 27 Shigella isolates (88.9%) showed multidrug resistance phenotypes to at least three classes of antimicrobials, The multidrug-resistant Shigella spp. were as follows: Shigella flexneri (66.7%), Shigella sonnei (18.5%), and Shigella dysenteriae (3.7%). The highest resistance was to streptomycin (100.0%), then to kanamycin (95.8%), nalidixic acid (95.8%), tetracycline (95.8%), spectinomycin (93.6%), ampicillin (87.5%), and sulfamethoxazole/trimethoprim (87.5%). PCR and DNA sequencing were used to screen and characterize integrons and antibiotic resistance genes. Our results indicated that 11.1% and 74.1% of isolates were positive for class 1 and class 2 integrons, respectively. Beta-lactamase-encoding genes were identified in 77.8% of isolates, and plasmid-mediated quinolone resistance genes were identified in 44.4% of isolates. These data provide useful information to better understand the molecular basis of antimicrobial resistance in Shigella spp. To the best of our knowledge, this is the first report of the molecular characterization of antibiotic resistance in Shigella spp. isolated from food. (C) 2014 Elsevier B.V. All rights reserved.