Pyrethroid and DDT cross-resistance in Aedes aegypti is correlated with novel mutations in the voltage-gated sodium channel gene
Pyrethroid and DDT cross-resistance in Aedes aegypti is correlated with novel mutations in the voltage-gated sodium channel gene
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DOI：
10.1046/j.1365-2915.2003.00412.x
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发表时间：
2003-03-01
影响因子：
1.9
通讯作者：
Hemingway, J
中科院分区：
文献类型：
--
作者：
Brengues, C;Hawkes, NJ;Hemingway, J
登革热病媒蚊埃及伊蚊（Aedes aegypti（L.）1995 ~ 1998年，从13个地区采集了10种蚊种，其中蚊种10种，双翅目：蚊科。两个实验室菌株，Bora（法属波利尼西亚）和AEAE，都对滴滴涕和氯菊酯敏感;所有其他菌株，除了Larentuka（印度尼西亚）和Bouake（象牙海岸），包含个别四龄幼虫抗氯菊酯。对10种菌株进行了一系列生化测定。与Bora菌株相比，许多菌株的羧酸酯酶活性升高;印度尼西亚菌株Salatiga和塞马朗以及Guyane菌株（卡宴）的羧酸酯酶活性尤其高。Salatiga和Paea（波利尼西亚）菌株中的单加氧酶水平增加，而两种泰国菌株（Mae Kaza，Mae库德）和Larentuka菌株中的单加氧酶水平降低。谷氨酰胺S-转移酶活性升高的Guyane菌株。所有其他酶谱与敏感菌株相似。在塞马朗、贝伦（巴西）和龙和（越南）菌株中同时存在滴滴涕和拟除虫菊酯抗性，表明存在敲低抗性（kdr）型抗性机制。用RT-PCR方法从13个田间品系的几种昆虫中获得了电压门控钠通道基因结构域II的部分S6疏水片段，并进行了测序。发现了四种新的突变。三个菌株在两个位置处含有相同的氨基酸取代，两个菌株共享不同的取代，并且一个菌株对于第四个改变是纯合的。亮氨酸苯丙氨酸取代，赋予神经不敏感性拟除虫菊酯在一系列其他抗性昆虫是不存在的。直接的神经生理学测定，从三个菌株与这些突变的个体幼虫表现出减少神经敏感性氯菊酯或λ氯氟氰菊酯抑制相比，敏感菌株。
Samples of the dengue vector mosquito Aedes aegypti (L.) (Diptera: Culicidae) were collected from 13 localities between 1995 and 1998. Two laboratory strains, Bora (French Polynesia) and AEAE, were both susceptible to DDT and permethrin; all other strains, except Larentuka (Indonesia) and Bouake (Ivory Coast), contained individual fourth-instar larvae resistant to permethrin. Ten strains were subjected to a range of biochemical assays. Many strains had elevated carboxylesterase activity compared to the Bora strain; this was particularly high in the Indonesian strains Salatiga and Semarang, and in the Guyane strain (Cayenne). Monooxygenase levels were increased in the Salatiga and Paea (Polynesia) strains, and reduced in the two Thai strains (Mae Kaza, Mae Kud) and the Larentuka strain. Glutathione S-transferase activity was elevated in the Guyane strain. All other enzyme profiles were similar to the susceptible strain. The presence of both DDT and pyrethroid resistance in the Semarang, Belem (Brazil) and Long Hoa (Vietnam) strains suggested the presence of a knock-down resistant (kdr)-type resistance mechanism. Part of the S6 hydrophobic segment of domain II of the voltage-gated sodium channel gene was obtained by RT-PCR and sequenced from several insects from all 13 field strains. Four novel mutations were identified. Three strains contained identical amino acid substitutions at two positions, two strains shared a different substitution, and one strain was homozygous for a fourth alteration. The leucine to phenylalanine substitution that confers nerve insensitivity to pyrethroids in a range of other resistant insects was absent. Direct neurophysiological assays on individual larvae from three strains with these mutations demonstrated reduced nerve sensitivity to permethrin or lambda cyhalothrin inhibition compared to the susceptible strains.