Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae)
Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae)
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DOI：
10.1002/aps3.1163
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发表时间：
2018-06
影响因子：
3.6
通讯作者：
Rui-Hong Wang;Mengdi Li;X. Wu;Chao Shen;Wendi Yu;Jinliang Liu;Zhechen Qi;Pan Li
中科院分区：
文献类型：
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作者：
Rui-Hong Wang;Mengdi Li;X. Wu;Chao Shen;Wendi Yu;Jinliang Liu;Zhechen Qi;Pan Li
本研究的前提是为北美东部广泛分布的林下藤本植物圆叶土豆(Smilax Rotondifolia)开发微卫星标记，研究其遗传多样性和遗传结构。在三个同属种中进行了交叉扩增：S.中国、S.riparia和S.walteri。方法和结果从圆叶沙参重新组装的转录组数据(88,3962个重叠群)中检测到6153对简单序列重复序列。从代表该物种4个自然居群的96个个体中选择了33个多态微卫星座位进行进一步分析。扩增出的等位基因数在2~15个之间，87.9%的扩增片段在3个同属种中至少有1个发生交叉扩增。结论本研究建立的简单序列重复标记为进一步研究圆叶土豆的遗传多样性和系统地理格局奠定了基础，并为其他物种提供了潜在的微卫星资源。
Premise of the Study Microsatellite markers were developed for Smilax rotundifolia (Smilacaceae), an understory vine widely distributed in eastern North America, to investigate genetic diversity and structure. Cross‐amplification was tested in three congeneric species: S. china, S. riparia, and S. walteri. Methods and Results A total of 6153 simple sequence repeat primer pairs were detected from the de novo–assembled transcriptome data (88,3962 contigs) of S. rotundifolia. Thirty‐three polymorphic microsatellite loci were selected for further analysis among 96 individuals representing four natural populations of the species. The number of alleles ranged from two to 15, and 87.9% of the developed primer pairs could be cross‐amplified in at least one of three congeneric Smilax species. Conclusions The simple sequence repeat markers developed in this study will facilitate further studies on genetic diversity and phylogeographic patterns of S. rotundifolia and provide additional potential microsatellite resources for other Smilax species.