ENHANCEMENT OF ACETYLCHOLINE-RELEASE DURING PARADOXICAL SLEEP IN THE DORSAL TEGMENTAL FIELD OF THE CAT BRAIN-STEM
ENHANCEMENT OF ACETYLCHOLINE-RELEASE DURING PARADOXICAL SLEEP IN THE DORSAL TEGMENTAL FIELD OF THE CAT BRAIN-STEM
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DOI：
10.1016/0304-3940(90)90576-u
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发表时间：
1990-07-13
影响因子：
2.5
通讯作者：
HONDA, Y
中科院分区：
文献类型：
--
作者：
KODAMA, T;TAKAHASHI, Y;HONDA, Y
脑干中的乙酰胆碱（ACh）与矛盾睡眠（PS）的产生有关。为了明确6只猫背部被膜场（FTD）局部乙酰胆碱释放（可能的ps产生位点）与睡眠-觉醒状态之间的关系，采用体内微透析和高效液相色谱-电化学检测方法测定乙酰胆碱。值得注意的是，在FTD中，乙酰胆碱释放量在PS期间比慢波睡眠和清醒时高约2倍（P < 0.001），但在尾状核（一个控制区）没有。脑脊液中乙酰胆碱释放似乎在PS发作前开始增加。电和化学（谷氨酸）刺激大细胞核（MC）增强了脑脊液中乙酰胆碱释放，缩短了PS潜伏期。这些结果表明，这种与ps相关的FTD中乙酰胆碱释放的增强是由MC中谷氨酸接受神经元的一些胆碱能投射引起的。
Acetylcholine (ACh) in the brain stem has been implicated in the generation of paradoxical sleep (PS). In order to clarify the relationship between local ACh release in the dorsal tegemental field (FTD), a possible PS-generating locus, and sleep-wake states in 6 cats, ACh was measured by the method of in vivo microdialysis and high performance liquid chromatography-electromchemical detection. It is noteworthy that ACh release was about 2 times higher (P < 0.001) during PS than during slow-wave sleep and wakefulness in FTD, but not in the caudate nucleus, a control region. ACh release in FTD appeared to begin to increase prior to the onset of PS. Electrical and chemical (glutamate) stimulations of the nucleus magnocellularis (MC) enhanced ACh release in FTD and shortened PS latency. These results suggest that this PS-related enhancement of ACh release in FTD is induced by some cholinergic projections from glutamate-receptive neurons in MC.