Extracellular vesicle miRNA-21 is a potential biomarker for predicting chronic lung disease in premature infants
Extracellular vesicle miRNA-21 is a potential biomarker for predicting chronic lung disease in premature infants
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DOI：
10.1152/ajplung.00166.2019
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发表时间：
2020-05-01
影响因子：
4.9
通讯作者：
Dennery, Phyllis A.
中科院分区：
文献类型：
--
作者：
Go, Hayato;Maeda, Hajime;Dennery, Phyllis A.
早产儿经常暴露于正压通气和补充氧气，这导致慢性肺部疾病（CLD）的发展。目前还没有标准的血清生物标志物用于预测或早期检测继续发展CLD的患者MicroRNA（miRNA）是一类新型的天然存在的短的非编码物质，其在转录后水平调节基因表达并引起翻译抑制和/或mRNA降解，并且存在于包装在细胞外囊泡（EV）中的体液中，使其非常稳定。我们的目的是评估在早产儿血清EV中鉴定的miRNA作为CLD的潜在生物标志物。从出生时和出生后第28天（DOL）的早产儿中提取血清EV。使用人类miRNA阵列。我们鉴定了62种在CLD患者和非CLD患者中普遍表达的miRNA。在62种miRNA中，CLD和非CLD患者中分别有59种和44种miRNA在DOLO和DOL28上差异表达。在这些miRNA中，与出生时的水平相比，DOL28时CLD患者的血清EV miR-21上调，而与出生时的水平相比，DOL28时非CLD患者的血清EV miR-21下调。在高氧暴露7天的新生小鼠中，作为CLD模型，5种miRNAs（miR-34a，miR-21. miR-712、miR-682和miR-221）上调。和7种miRNAs（miR-542 - 5p、miR-449a、miR-322、miR-190b）。miR-153、miR-335 - 3p、miR-377）下调。miR-21是一种常见的miRNA，在CID患者和高氧暴露小鼠中发生变化。结论EV miR-21可能是CLD的生物标志物。
Premature infants are often exposed to positive pressure ventilation and supplemental oxygen, which leads to the development of chronic lung disease (CLD). There are currently no standard serum biomarkers used for prediction or early detection of patients who go on to develop CLD MicroRNAs (miRNAs) are a novel class of naturally occurring, short, noncoding substances that regulate gene expression at the posttranscriptional level and cause translational inhibition and/or mRNA degradation and present in body fluids packaged in extracellular vesicles (EVs), rendering them remarkably stable. Our aim was to evaluate miRNAs identified in serum EVs of premature infants as potential biomarkers for CLD. Serum EVs were extracted from premature infants at birth and on the 28th day of life (DOL). Using a human miRNA array. we identified 62 miRNAs that were universally expressed in CLD patients and non-CLD patients. Of the 62 miRNAs, 59 miRNAs and 44 miRNAs were differentially expressed on DOLO and DOL28 in CLD and non-CLD patients, respectively. Of these miRNAs, serum EV miR-21 was upregulated in CLD patients on DOL28 compared with levels at birth and downregulated in non-CLD patients on DOL28 compared with levels at birth. In neonatal mice exposed to hyperoxia for 7days, as a model of CLD, five miRNAs (miR-34a, miR-21. miR-712, miR-682, and miR-221) were upregulated. and 7 miRNAs (miR-542-5p, miR-449a, miR-322, miR-190b. miR-153, miR-335-3p, miR-377) were downregulated. MiR-21 was detected as a common miRNA that changed in CID patients and in the hyperoxia exposed mice. We conclude that EV miR-21 may be a biomarker of CLD.