Gold and Hairpin DNA Functionalization of Upconversion Nanocrystals for Imaging and In Vivo Drug Delivery
Gold and Hairpin DNA Functionalization of Upconversion Nanocrystals for Imaging and In Vivo Drug Delivery
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DOI：
10.1002/adma.201700244
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发表时间：
2017-05-10
影响因子：
29.4
通讯作者：
Liu, Xiaogang
中科院分区：
文献类型：
--
作者：
Han, Sanyang;Samanta, Animesh;Liu, Xiaogang
尽管多功能上转换成像探针最近在生物医学研究中引起了相当大的兴趣，但目前在生物系统中用寡核苷酸稳定这些发光纳米探针的方法很少。本文中，提出了一种基于包含发夹DNA修饰的金纳米颗粒的纳米缀合物的合理设计和合成来将上转换纳米探针稳健地分散在生理缓冲液中的方法。这种方法赋予上转换纳米探针优异的生物相容性，并避免了颗粒团聚的问题。通过结合单波段反斯托克斯近红外发射和光热效应介导的耦合金的上转换纳米粒子，一个简单的，通用的纳米粒子系统，同时在体内的深部组织成像和药物分子释放的证明。
Although multifunctional upconversion imaging probes have recently attracted considerable interest in biomedical research, there are currently few methods for stabilizing these luminescent nanoprobes with oligonucleotides in biological systems. Herein, a method to robustly disperse upconversion nanoprobes in physiological buffers based on rational design and synthesis of nanoconjugates comprising hairpin-DNA-modified gold nanoparticles is presented. This approach imparts the upconversion nanoprobes with excellent biocompatibility and circumvents the problem of particle agglomeration. By combining single-band anti-Stokes near-infrared emission and the photothermal effect mediated by the coupling of gold to upconversion nanoparticles, a simple, versatile nanoparticulate system for simultaneous deep-tissue imaging and drug molecule release in vivo is demonstrated.