A deep redox proteome profiling workflow and its application to skeletal muscle of a Duchenne Muscular Dystrophy model.
A deep redox proteome profiling workflow and its application to skeletal muscle of a Duchenne Muscular Dystrophy model.
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DOI：
10.1016/j.freeradbiomed.2022.10.300
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发表时间：
2022-11-20
影响因子：
7.4
通讯作者：
Qian, Wei-Jun
中科院分区：
文献类型：
--
作者：
Day, Nicholas J.;Zhang, Tong;Gaffrey, Matthew J.;Zhao, Rui;Fillmore, Thomas L.;Moore, Ronald J.;Rodney, George G.;Qian, Wei-Jun
关键词：
氧化还原状态的扰动伴随着许多疾病，其影响可以通过生物分子（包括蛋白质、脂质和核酸）的氧化来观察。蛋白质半胱氨酸残基的硫醇基团经历一系列氧化还原翻译后修饰 (PTM)，这对于蛋白质和通路功能的调节非常重要。为了更好地了解哪些蛋白质在扰动后受到氧化还原调节，重要的是能够在蛋白质组水平上全面分析蛋白质硫醇氧化。在此，我们报告了深度氧化还原蛋白质组分析工作流程，并展示了其在测量杜氏肌营养不良症 (DMD) 模型 mdx 小鼠骨骼肌中硫醇氧化变化和整体蛋白质表达方面的应用。通过对肌肉中 5,608 个蛋白质的超过 18,000 个 Cys 位点进行定量，实现了硫醇蛋白质组的深度覆盖。与对照组相比，mdx 小鼠表现出显着增加的硫醇氧化，其中观察到中位氧化占有率发生约 2% 的变化。氧化还原数据的通路分析显示，凝血系统和免疫相关通路是 mdx 小鼠中最容易受到硫醇氧化增加影响的通路之一，而蛋白质丰度变化在与生物能学相关的通路中更为丰富。这项研究说明了深度氧化还原分析对于更深入地了解氧化应激调节和氧化环境中受到干扰的途径/过程的重要性。
Perturbation to the redox state accompanies many diseases and its effects are viewed through oxidation of biomolecules, including proteins, lipids, and nucleic acids. The thiol groups of protein cysteine residues undergo an array of redox post-translational modifications (PTMs) that are important for regulation of protein and pathway function. To better understand what proteins are redox regulated following a perturbation, it is important to be able to comprehensively profile protein thiol oxidation at the proteome level. Herein, we report a deep redox proteome profiling workflow and demonstrate its application in measuring the changes in thiol oxidation along with global protein expression in skeletal muscle from mdx mice, a model of Duchenne Muscular Dystrophy (DMD). In-depth coverage of the thiol proteome was achieved with >18,000 Cys sites from 5,608 proteins in muscle being quantified. Compared to the control group, mdx mice exhibit markedly increased thiol oxidation, where a ~2% shift in the median oxidation occupancy was observed. Pathway analysis for the redox data revealed that coagulation system and immune-related pathways were among the most susceptible to increased thiol oxidation in mdx mice, whereas protein abundance changes were more enriched in pathways associated with bioenergetics. This study illustrates the importance of deep redox profiling in gaining greater insight into oxidative stress regulation and pathways/processes that are perturbed in an oxidizing environment.
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胶原蛋白VI的消融导致功能变化的血小板释放。
影响因子：
7.5
作者：
Abbonante, Vittorio;Gruppi, Cristian;Battiston, Monica;Zulian, Alessandra;Di Buduo, Christian Andrea;Chrisam, Martina;Sereni, Lucia;Laurent, Pierre-Alexandre;Semplicini, Claudio;Lombardi, Elisabetta;Mazzucato, Mario;Moccia, Francesco;Petronilli, Valeria;Villa, Anna;Bello, Luca;Pegoraro, Elena;Bernardi, Paolo;Braghetta, Paola;De Marco, Luigi;Bonaldo, Paolo;Balduini, Alessandra
通讯作者：
Balduini, Alessandra
DOI：
10.3390/antiox10030499
发表时间：
2021-03-23
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
Day NJ;Gaffrey MJ;Qian WJ
通讯作者：
Qian WJ
DOI：
10.3791/62671
发表时间：
2021-06-21
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Gaffrey MJ;Day NJ;Li X;Qian WJ
通讯作者：
Qian WJ
影响因子：
14.8
作者：
通讯作者：
--
影响因子：
11.4
作者：
Cully, Tanya R.;Rodney, George G.
通讯作者：
Rodney, George G.