Hepatocellular carcinoma-associated gene 2 interacts with MAD2L2
Hepatocellular carcinoma-associated gene 2 interacts with MAD2L2
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DOI：
10.1007/s11010-007-9512-8
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发表时间：
2007-10-01
影响因子：
4.3
通讯作者：
Huo, Keke
中科院分区：
文献类型：
--
作者：
Li, Li;Shi, Yan;Huo, Keke
HCCA2（肝细胞癌相关基因2）最初被确定为HCC（肝细胞癌）特异性蛋白，随后，HCCA2的长剪接变体被确定为转录因子YY1（阴阳1）的共激活因子。为了研究HCCA2在HCC发生和发展中的作用，我们筛选了一个人胎儿肝脏cDNA文库，并鉴定了一个新的HCCA2相互作用蛋白MAD2L2 （MAD2有丝分裂停止缺陷样2（酵母））。体外和体内结合实验证实了HCCA2与MAD2L2的相互作用，并通过序列删除将相互作用结构域定位到HCCA2的n端。HCCA2和MAD2L2也存在于Hela细胞的细胞核中。此外，过表达HCCA2导致细胞周期阻滞在G0/G1期，从而抑制细胞增殖。我们的研究表明，HCCA2可能在细胞周期调节中发挥新的作用。
HCCA2 (hepatocellular carcinoma-associated gene 2) was initially identified as a HCC (hepatocellular carcinoma)-specific protein and subsequently, a long splice variant of HCCA2 was identified as a co-activator of transcription factor YY1 (Yin Yang 1). To investigate the role of HCCA2 in HCC genesis and progression, we screened a human fetal liver cDNA library and identified a novel HCCA2-interacting protein, MAD2L2 (MAD2 mitotic arrest deficient-like 2 (yeast)). The interaction between HCCA2 and MAD2L2 was confirmed by in vitro and in vivo binding assays and the interaction domain was mapped to the N-terminus of HCCA2 by sequential deletion. HCCA2 and MAD2L2 also colocalized in the nucleus of Hela cells. Furthermore, overexpression of HCCA2 led to cell cycle arrest at G0/G1 phase and therefore inhibited cell proliferation. Our research suggests that HCCA2 may play a novel role in cell cycle regulation.