MOLECULES RELEVANT FOR T-CELL-TARGET CELL-INTERACTION ARE PRESENT IN CYTOLYTIC GRANULES OF HUMAN LYMPHOCYTES-T
MOLECULES RELEVANT FOR T-CELL-TARGET CELL-INTERACTION ARE PRESENT IN CYTOLYTIC GRANULES OF HUMAN LYMPHOCYTES-T
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DOI：
10.1002/eji.1830190819
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发表时间：
1989-08-01
影响因子：
5.4
通讯作者：
BORST, J
中科院分区：
文献类型：
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作者：
PETERS, PJ;GEUZE, HJ;BORST, J
人细胞毒性T淋巴细胞-靶细胞（CTL-TC）相互作用的超微结构分析已进行，使更好地了解杀伤机制。人们的注意力集中在CTL中的颗粒上，已知这些颗粒含有致命的化合物。在膜界定的细胞毒性颗粒的电子致密的核心，以及许多膜囊泡被确定。在CTL-TC缀合物中，发生特异性膜相互作用，允许形成细胞间裂缝，颗粒核心和内部囊泡被释放到其中。已知参与CTL-TC相互作用的T细胞表面膜分子（T细胞受体、CD 3和CD 8）存在于颗粒核心和内部囊泡的膜上，面向外。对这种膜定位的解释可能是颗粒与内吞途径有关。此外，颗粒的内腔富含组织蛋白酶D，这表明与溶酶体隔室相关。可见胞吐的囊泡和核心粘附在TC的质膜上。虽然颗粒囊泡和核心的确切内容仍有待确定，我们建议，特异性相互作用的CTL膜分子上的细胞溶解颗粒成分与分子上的质膜的TC可以确保单向传递的致命打击。
An ultrastructural analysis of human cytotoxic T lymphocyte-target cell (CTL-TC) interaction has been undertaken to enable a better understanding of the killing mechanism. Attention was focused on granules in the CTL, which are known to contain lethal compounds. Within the membrane-delimited cytotoxic granule an electron-dense core as well as numerous membrane vesicles were identified. In CTL-TC conjugates, specific membrane interactions take place, allowing the formation of intercellular clefts into which the granule cores and internal vesicles are released. T cell surface membrane molecules known to be involved in CTL-TC interaction (T cell receptor, CD3 and CD8) are present on the membranes of the granule cores and internal vesicles, facing outward. An explanation for this localization of the membrane may be found in the fact that the granule is connected with an endocytotic pathway. Moreover, the lumen of the granule is rich in the enzyme cathepsin D, which indicates an association with a lysosomal compartment. Exocytosed vesicles and cores are seen to adhere to the plasma membrane of the TC. Although the exact contents of the granule vesicles and core remain to be identified, we suggest that specific interaction of CTL membrane molecules on the cytolytic granule components with molecules on the plasma membrane of the TC may ensure the unidirectional delivery of the lethal hit.