Spray dried human and chimpanzee adenoviral-vectored vaccines are thermally stable and immunogenic in vivo
Spray dried human and chimpanzee adenoviral-vectored vaccines are thermally stable and immunogenic in vivo
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DOI：
10.1016/j.vaccine.2017.04.026
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发表时间：
2017-05-19
期刊：
影响因子：
5.5
通讯作者：
Xing, Zhou
中科院分区：
文献类型：
--
作者：
Afkhami, Sam;LeClair, Daniel A.;Xing, Zhou
需要无冷链疫苗技术来确保有效的疫苗交付和覆盖率，特别是在资源贫乏的国家。然而，喷雾干燥的活病毒载体疫苗如重组腺病毒载体疫苗的免疫原性和热稳定性仍有待研究。为了解决这个问题，我们在甘露醇和葡聚糖基质中喷雾干燥了人腺病毒（AdHu5）和黑猩猩腺病毒（AdCh68）载体的结核病疫苗。含有这两种疫苗的喷雾干燥粉末显示出长期热稳定性和疫苗接种所需的形态学和化学性质。重构后，它们在体外有效地转染细胞，与喷雾干燥过程相关的病毒感染性损失相对较小。体内接种后，AdHu5和AdCh68载体疫苗的免疫原性与常规新鲜冷冻保存的液体疫苗样品一样。重要的是，即使在无冷链储存后，在环境温度和相对低的湿度下储存30和90天，疫苗仍保留其体内免疫原性，而在相同条件下储存的液体疫苗样品几乎完全丧失其免疫活化能力。我们的研究结果支持我们的喷雾干燥技术的进一步发展，以产生热稳定的腺病毒载体和其他病毒载体的疫苗。(C)2017爱思唯尔有限公司版权所有
Cold chain-free vaccine technologies are needed to ensure effective vaccine delivery and coverage, particularly in resource-poor countries. However, the immunogenicity and thermostability of spray dried live viral vector-based vaccines such as recombinant adenoviral-vectored vaccines remain to be investigated. To address this issue, we have spray dried human adenoviral (AdHu5)- and chimpanzee adenoviral (AdCh68)-vectored tuberculosis vaccines in a mannitol and dextran matrix. Spray dried powders containing these two vaccines display the morphologic and chemical properties desired for long-term thermostability and vaccination. Upon reconstitution, they effectively transfected the cells in vitro with relatively small losses in viral infectivity related to the spray drying process. Following in vivo vaccination, AdHu5- and AdCh68-vectored vaccines were as immunogenic as the conventional fresh, cryopreserved liquid vaccine samples. Of importance, even after cold chain-free storage, at ambient temperatures and relatively low humidity for 30 and 90 days, the vaccines retained their in vivo immunogenicity, while the liquid vaccine samples stored under the same conditions lost their immune-activating capability almost entirely. Our results support further development of our spray drying technologies for generating thermally stable adenoviral-vectored and other viral-vectored vaccines. (C) 2017 Elsevier Ltd. All rights reserved.