Identification of signals required for the insertion of heterologous genome segments into the reovirus genome.
Identification of signals required for the insertion of heterologous genome segments into the reovirus genome.
复制标题
鉴定将异源基因组片段插入呼肠孤病毒基因组所需的信号。
DOI：
10.1073/pnas.92.26.12362
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发表时间：
1995
影响因子：
11.1
通讯作者：
Joklik,WK
中科院分区：
文献类型：
--
作者：
Roner,MR;Lin,PN;Nepluev,I;Kong,LJ;Joklik,WK
在同时感染三种呼肠孤病毒血清型ST1、ST2和ST3中的任何两种的细胞中，高达15%的产量是包含亲本基因组片段所有可能组合的型间重组。我们现在已经发现，并不是所有的重组基因组片段都是野生型。在具有比ST3基因组片段更多的ST1基因组片段的重组中，所有ST1基因组片段似乎都是野生型，但传入的ST3基因组片段具有突变，使它们与它们所取代的ST1基因组片段更相似。在具有大部分ST3基因组片段的远亲ST3和ST2病毒杂交产生的重组中，所有传入的ST2基因组片段都是野生型，但ST3 S4基因组片段具有两个突变，G74到a和G624到a，作为接受信号。识别这些信号对构建具有新特性和功能的呼肠孤病毒具有深远的意义。
In cells simultaneously infected with any two of the three reovirus serotypes ST1, ST2, and ST3, up to 15% of the yields are intertypic reassortants that contain all possible combinations of parental genome segments. We have now found that not all genome segments in reassortants are wild type. In reassortants that possess more ST1 than ST3 genome segments, all ST1 genome segments appear to be wild type, but the incoming ST3 genome segments possess mutations that make them more similar to the ST1 genome segments that they replace. In reassortants resulting from crosses of the more distantly related ST3 and ST2 viruses that possess a majority of ST3 genome segments, all incoming ST2 genome segments are wild type, but the ST3 S4 genome segment possesses two mutations, G74 to A and G624 to A, that function as acceptance signals. Recognition of these signals has far-reaching implications for the construction of reoviruses with novel properties and functions.