Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli
Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli
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DOI：
10.1016/j.ab.2005.07.002
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发表时间：
2005-10-15
影响因子：
2.9
通讯作者：
Raetz, CRH
中科院分区：
文献类型：
--
作者：
Guan, ZQ;Breazeale, SD;Raetz, CRH
从大肠杆菌细胞中提取十一戊烯基二磷酸- murnac -五肽- glcnac（脂质II），利用其在bligher - dyer系统中不寻常的ph依赖性溶解度特性，并通过电喷雾电离质谱法与新型N-15质移分析相结合进行鉴定。所描述的方法将促进来自不同细菌的脂质11变体的结构表征，包括抗生素耐药突变体，以及所有生物系统中存在的许多次要未表征的脂质。(C) 2005爱思唯尔公司版权所有。
Undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc (lipid II) is extracted from Escherichia coli cells by utilizing its unusual pH-dependent solubility property in a Bligh-Dyer system, and identified by electrospray ionization mass spectrometry in conjunction with a novel N-15 mass shift analysis. The described approach will facilitate the structural characterization of lipid 11 variants from diverse bacteria, including antibiotic-resistant mutants, as well as the numerous minor uncharacterized lipids present in all biological systems. (C) 2005 Elsevier Inc. All rights reserved.