Up-regulated transcripts in a compatible powdery mildew-grapevine interaction
Up-regulated transcripts in a compatible powdery mildew-grapevine interaction
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DOI：
10.1016/j.plaphy.2009.03.006
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发表时间：
2009-08-01
影响因子：
6.5
通讯作者：
Kovacs, Laszlo G.
中科院分区：
文献类型：
--
作者：
Fekete, Csaba;Fung, Raymond W. M.;Kovacs, Laszlo G.
白粉病（Erysiphales）是专性生物营养病原体，侵入易感植物细胞而不引发细胞死亡。这表明了一种高度熟练的寄生机制，使白粉病能够避免被宿主发现或逃避防御。为了更好地了解这种植物与病原体的相互作用，我们采用抑制减法杂交（SSH）、差分杂交（differential hybridization）和定量实时荧光定量PCR （qRT）技术鉴定了葡萄（Vitis vinifera L.）对葡萄白粉病Erysiphe necator Schwein的特异性上调基因。我们鉴定了25个葡萄转录本，在易感寄主V vinifera Cabernet Sauvignon的叶片中感染后丰度增加。尽管病原菌和植物之间的互作是相容的，但一些赤霉病菌诱导的转录本代表了典型的防御反应基因。在鉴定的转录本中，编码了富含亮氨酸的重复丝氨酸/苏氨酸激酶样受体、MYB转录因子和两个泛素化相关蛋白的转录本，表明细胞内信号转导和调节功能的刺激。还鉴定了一些衰老过程特征基因，包括金属硫蛋白、脱氧核糖核酸酶、天冬氨酸蛋白酶和枯草酶样丝氨酸蛋白酶。这些转录本扩展了先前鉴定的葡萄球菌响应基因列表，并促进了对这种经济上重要的植物-病原体相互作用的分子事件的更全面的看法。(C) 2009 Elsevier Masson SAS。版权所有。
Powdery mildews (Erysiphales) are obligate biotrophic pathogens that invade susceptible plant cells without triggering cell death. This suggests a highly adept mechanism of parasitism which enables powdery mildews to avoid detection or evade defenses by their host. To better understand this plant-pathogen interaction, we employed suppression subtractive hybridization (SSH), differential hybridization and quantitative real-time (qRT) PCR for the identification of grapevine (Vitis vinifera L.) genes that were specifically up-regulated in response to the grape powdery mildew Erysiphe necator Schwein. We identified 25 grapevine transcripts that increased in abundance upon infection in leaves of the susceptible host V vinifera Cabernet Sauvignon. Despite the compatible interaction between the pathogen and plant, several of the E. necator-induced transcripts represented typical defense response genes. Among the transcripts identified were those that encoded a leucine-rich repeat serine/threonine kinase-like receptor, an MYB transcription factor, and two ubiquitination-associated proteins, indicating the stimulation of intracellular signal transduction and regulatory functions. A number of genes characteristic of senescence processes, including metallothioneins, a deoxyribonuclease, an aspartyl protease and a subtilase-like serine protease, also were identified. These transcripts expanded the list of previously identified E. necator-responsive grapevine genes and facilitated a more comprehensive view of the molecular events that underlie this economically important plant-pathogen interaction. (C) 2009 Elsevier Masson SAS. All rights reserved.