Resistance Performances of Transgenic Bt Rice Lines T2A-1 and T1c-19 Against Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
Resistance Performances of Transgenic Bt Rice Lines T2A-1 and T1c-19 Against Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
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DOI：
10.1603/ec10389
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发表时间：
2011-10
期刊：
影响因子：
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通讯作者：
Xu-song Zheng;Yajun Yang;Hong-xing Xu;Hao Chen;Baoju Wang;Yongjun Lin;Zhongxian Lu
中科院分区：
文献类型：
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作者：
Xu-song Zheng;Yajun Yang;Hong-xing Xu;Hao Chen;Baoju Wang;Yongjun Lin;Zhongxian Lu
摘要转Bt基因水稻，采用离体叶片生物测定法，对表达Cry 2A * 和Cry 1C * 的闽恢63品系T2 A-1和T1 c-19对稻纵卷叶螟（Cnaphalocrocis medinalis）初孵幼虫和3龄幼虫的抗性进行了评价。T2 A-1和T1 c-19水稻均表现出较高的C.内侧阻力;而T2 A-1水稻的半数致死时间（LT 50）显著长于T1 c-19水稻，表明T1 c-19水稻对稻曲病的毒力更强。纵卷蛾幼虫幼虫死亡率4天后，无氮MH 63为25.5%，而高氮肥（250公斤N/公顷）植物的死亡率为2.4%。与无氮植物相比，高氮T2 A-1植物上的幼虫死亡率下降了20%。然而，T1 c-19植物的抗性不受氮肥的影响。C.稻纵卷叶螟在苗期和灌浆期都喜欢MH 63产卵，而不喜欢T1 c-19和T2 A-1 Bt水稻品系。
ABSTRACT The transgenic Bacillus thuringiensis (Bt) rice, Oryza sativa L., lines T2A-1 and T1c-19 expressing Cry2A* and Cry1C* from ‘Minhui 63’ (MH63) were evaluated for resistance to newly hatched and third-instar larvae of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), by using detached leaf laboratory bioassays. Both T2A-1 and T1c-19 rice showed high C. medinalis resistance; however, the lethal time (LT)50 of larvae fed with T2A-1 rice was significantly longer than that of larvae fed with T1c-19 rice, implying T1c-19 rice was more toxic to C. medinalis larvae. Larval mortality after 4 d on nitrogen-free MH63 was 25.5% compared with 2.4% mortality on high nitrogen fertilizer (250 kg N/ha) plants. Larval mortality on high nitrogen T2A-1 plants declined by 20% compared with nitrogen-free plants. However, resistance in T1c-19 plants was unaffected by nitrogen fertilizer. C. medinalis moths preferred MH63 at both the seedling and grain milk stages for oviposition but not the T1c-19 and T2A-1 Bt rice lines.