Effects of cyclosporin-A-induced immunosuppression on periapical lesions in rats
Effects of cyclosporin-A-induced immunosuppression on periapical lesions in rats
复制标题
DOI：
10.1177/154405910408300905
复制
发表时间：
2004-09-01
影响因子：
7.6
通讯作者：
Ebisu, S
中科院分区：
文献类型：
--
作者：
Kawahara, T;Murakami, S;Ebisu, S
环孢素 A (CsA) 可能会诱导根尖周病变的免疫反应改变并改变骨重塑。本研究确定了 CsA 给药期间和停药后大鼠根尖周病变发生的变化。诱导根尖损伤后，用CsA（0-20mg/kg/天）治疗动物4周。通过计算机断层扫描测量病变体积。使用抗CD3和抗CD25抗体进行组织学观察和免疫组织化学评估。 CsA 给药减少了病灶体积，并且 CsA 停药后病灶明显扩大。 CsA 抑制病变部位 T 细胞的增殖和活化。 CsA 对 T 细胞的影响呈剂量依赖性，最高剂量为 10 mg/kg/天，此后没有明显差异。这些结果表明，CsA 通过干扰根尖周病变中的 T 细胞功能来抑制根尖周破坏。
Cyclosporin A ( CsA) might induce immune response alterations in periapical lesions and modify bone remodeling. This study determined the changes that occur in the periapical lesions of rats during CsA administration and after CsA withdrawal. After the induction of periapical lesions, the animals were treated with CsA ( 0- 20 mg/ kg/ day) for 4 wks. Lesion volumes were measured by computed tomography. Histological observations and immunohistochemical evaluations were performed with anti- CD3 and anti- CD25 antibodies. CsA administration reduced lesion volumes, and the lesions significantly expanded after CsA withdrawal. CsA inhibited the proliferation and activation of T- cells at lesion sites. The effects of CsA on T- cells were dose-dependent up to 10 mg/ kg/ day, after which no significant difference was evident. These results suggest that CsA inhibits periapical destruction by interfering with T- cell function in periapical lesions.