Diversity of laccase-like multicopper oxidase genes in Morchellaceae:: identification of genes potentially involved in extracellular activities related to plant litter decay
Diversity of laccase-like multicopper oxidase genes in Morchellaceae:: identification of genes potentially involved in extracellular activities related to plant litter decay
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DOI：
10.1111/j.1574-6941.2007.00322.x
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发表时间：
2007-07-01
影响因子：
4.2
通讯作者：
Buscot, Francois
中科院分区：
文献类型：
--
作者：
Kellner, Harald;Luis, Patricia;Buscot, Francois
尽管土生子囊菌在植物凋落物腐烂过程中发挥了重要作用，但对其漆酶样多铜氧化酶(LMCO)基因的多样性和功能的研究还很少。采用聚合酶链式反应技术对9个羊肚菌科和1个豆科植物的15个菌株的LMCO基因多样性进行了分析。在该物种中发现了1至6个不同的基因，代表26种不同的序列类型。聚类分析表明，LMCO基因属于编码不同蛋白质类(第I-IV类)的4个主要基因家族。为了确定与细胞外活动相关并可能参与凋落过程的基因，用不同的芳香族化合物诱导液体培养。在天然酚类化合物愈创木酚的存在下，羊肚菌和锥叶蝉表现出最强的LMCO活性增强，并通过测序对其表达的LMCO基因进行了鉴定。只表达了编码第II类和第III类蛋白的基因家族的基因。类菌根菌株M.conica的两个基因(II类和III类)都在愈创木酚存在下唯一表达。与腐生型菌株不同，编码III类蛋白的基因在没有愈创木酚的对照培养中也得到了结构性表达。
Despite the important role played by soil-inhabiting ascomycetes in plant litter decay processes, studies on the diversity and function of their laccase-like multicopper oxidase (LMCO) genes are scarce. In the present work, the LMCO gene diversity in 15 strains representing nine Morchellaceae and one Discinaceae species was evaluated by PCR. One to six different genes were found within the species, representing 26 different sequence types. Cluster analysis revealed LMCO genes belonging to four main gene families encoding different protein classes (Class I-IV). To identify the genes related to extracellular activities and potentially involved in litter decay processes, liquid cultures were induced by different aromatic compounds. Morchella conica and Verpa conica showed the strongest LMCO activity enhancement in the presence of the naturally occurring phenolic compound guaiacol, and their expressed LMCO genes were identified by sequencing. Only genes belonging to the gene families encoding the Class II and III proteins were expressed. Both genes (Class II and III) of the mycorrhizal-like strain M. conica were exclusively expressed in the presence of guaiacol. In contrast to the saprotrophic strain V. conica, the gene encoding the Class III protein was constitutively expressed as it was also found in control cultures without guaiacol.