A cross-sectional study of paratuberculosis in 1155 Danish dairy cows
A cross-sectional study of paratuberculosis in 1155 Danish dairy cows
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DOI：
10.1016/s0167-5877(00)00138-0
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发表时间：
2000-07-03
影响因子：
2.6
通讯作者：
Nielsen, SS
中科院分区：
文献类型：
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作者：
Jakobsen, MS;Alban, L;Nielsen, SS
在一项对来自22个丹麦奶牛群的1155头奶牛的牛奶样本进行的横断面研究中，确定了副结核病的选定风险因素。所用的诊断程序是一种间接酶联免疫吸附试验（ELISA），以检测抗体的鸟分枝杆菌亚种。副结核病如果样本的校正光密度大于或等于0.025（检测灵敏度为71.4%，检测特异性为89.7%），则认为该样本为检测阳性。在1155份样本中，8.8%（102/1155）为静息阳性，22个奶牛群中有19个奶牛群的试验阳性奶牛数量大于或等于1头。多元Logistic回归分析中的显著风险因素为：泽西与大型品种，高产次与低产次，产犊后第一个月与其他哺乳期月份，以及大型牛群与小型牛群相比。在年龄较大的奶牛（胎次>4）中观察到阳性检测的概率最高（37-38%），并在产犊后第一个月内进行检测（不考虑品种）。在产犊前或产犊后一个月后检测的第一胎大品种奶牛中观察到阳性检测结果的概率最低（2%）。(C)2000 Elsevier Science B. V.保留所有权利。
In a cross-sectional study on milk samples from 1155 cows from 22 Danish dairy herds, selected risk factors for paratuberculosis were identified. The diagnostic procedure used was an indirect enzyme-linked immuno-sorbent assay (ELISA) to detect antibodies against Mycobacterium avium subsp. paratuberculosis. A sample was considered test-positive if it had a corrected optical density greater than or equal to 0.025 (test sensitivity 71.4% and test specificity 89.7%). Of the 1155 samples, 8.8% (102/1155) were rest-positive, and 19 out of the 22 dairy herds had greater than or equal to 1 test-positive cows. The significant risk factors in a multiple logistic regression analysis were: Jersey versus large breeds, high parity versus low parity, the first month after calving versus other months of lactation, and a large herd size compared to a small herd size. The highest probability (37-38%) of a positive test was observed among older cows (parity >4) and tested within the first month after calving (irrespective of breed). The lowest probability (2%) of a positive test-result was observed among first parity, large-breed cows tested before calving or later than one month after. (C) 2000 Elsevier Science B.V. All rights reserved.