GRIN lens applications for studying neurobiology of substance use disorder.
GRIN lens applications for studying neurobiology of substance use disorder.
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DOI：
10.1016/j.addicn.2022.100049
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发表时间：
2022-12-01
期刊：
影响因子：
--
通讯作者：
Lin, Da-Ting
中科院分区：
文献类型：
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作者：
Beacher, Nicholas James;Washington, Kayden Alecsandre;Lin, Da-Ting
物质使用障碍（SUD）与严重的健康和社会后果有关。持续的药物使用导致中脑边缘多巴胺系统内的回路改变。观察SUD对体内神经活动的纵向影响，以确定SUD易感性，开发药物以防止过量，并帮助患有SUD的人，这是至关重要的。然而，涉及的SUD相关区域埋藏在脑深部，这使得在体内观察神经活动具有挑战性。梯度折射率（GRIN）透镜可以探测小鼠和大鼠的这些区域。在这篇简短的综述中，我们将讨论GRIN透镜如何与其他技术（如微型显微镜、纤维镜、功能磁共振成像和光遗传学）相结合，以充分探索体内SUD研究。特别地，GRIN透镜允许在体内观察涉及SUD的脑深部区域、遗传上不同的神经元的分化、纵向检查单个细胞、神经元模式与SUD行为的相关性以及神经回路的操纵。
Substance use disorder (SUD) is associated with severe health and social consequences. Continued drug use results in alterations of circuits within the mesolimbic dopamine system. It is critical to observe longitudinal impacts of SUD on neural activity in vivo to identify SUD predispositions, develop pharmaceuticals to prevent overdose, and help people suffering from SUD. However, implicated SUD associated areas are buried in deep brain which makes in vivo observation of neural activity challenging. The gradient index (GRIN) lens can probe these regions in mice and rats. In this short communications review, we will discuss how the GRIN lens can be coupled with other technologies such as miniaturized microscopes, fiberscopes, fMRI, and optogenetics to fully explore in vivo SUD research. Particularly, GRIN lens allows in vivo observation of deep brain regions implicated in SUD, differentiation of genetically distinct neurons, examination of individual cells longitudinally, correlation of neuronal patters with SUD behavior, and manipulation of neural circuits.