Identification of SNPs and INDELS in swine transcribed sequences using short oligonucleotide microarrays
Identification of SNPs and INDELS in swine transcribed sequences using short oligonucleotide microarrays
复制标题
DOI：
10.1186/1471-2164-9-252
复制
发表时间：
2008-05-29
期刊：
影响因子：
4.4
通讯作者：
Piedrahita, Jorge A.
中科院分区：
文献类型：
--
作者：
Bischoff, Steve R.;Tsai, Shengdar;Piedrahita, Jorge A.
背景资料：通过比较梅山猪或西方猪与Affyellow猪微阵列的复合品种的探针强度，使用第25天胎盘RNA的转录组谱进行猪的单特征多态性（SFP）的全基因组检测。线性混合模型分析被用来确定显着的品种由probe interaction.Results：基因特异性线性混合模型拟合到这些阵列上的每个log（2）转换的探针强度，使用固定效应的品种，探针，品种由探针的相互作用，和阵列的随机效应。在调查第25天胎盘转录组后，857个探针的q值
Background: Genome-wide detection of single feature polymorphisms (SFP) in swine using transcriptome profiling of day 25 placental RNA by contrasting probe intensities from either Meishan or an occidental composite breed with Affymetrix porcine microarrays is presented. A linear mixed model analysis was used to identify significant breed-by-probe interactions.Results: Gene specific linear mixed models were fit to each of the log(2) transformed probe intensities on these arrays, using fixed effects for breed, probe, breed-by-probe interaction, and a random effect for array. After surveying the day 25 placental transcriptome, 857 probes with a q-value