Protein Secondary Structure Imaging with Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy
Protein Secondary Structure Imaging with Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy
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DOI：
10.1021/jp210914x
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发表时间：
2012-02-02
影响因子：
3.3
通讯作者：
Hamaguchi, Hiro-o
中科院分区：
文献类型：
--
作者：
Bito, Kotatsu;Okuno, Masanari;Hamaguchi, Hiro-o
利用超宽带多重相干反斯托克斯拉曼散射（汽车）显微光谱技术研究了人发蛋白质的二级结构。汽车峰移映射方法已被开发和应用到头发样品的化学还原和机械拉伸处理和不处理。它清楚地显示了处理诱导的蛋白质二级结构及其空间分布的变化。使用新的成像技术，我们在人类头发皮层中发现了多层结构。
Protein secondary structures in human hair have been studied with ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The CARS peak-shift mapping method has been developed and applied to hair samples with and without treatments by chemical reduction and mechanical extension. It clearly visualizes the treatment induced changes in protein secondary structures and their spatial distributions. Using the new imaging technique, we found a multilayered structure in the human hair cortex.