MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line
MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line
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DOI：
10.3892/ol.2017.6348
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发表时间：
2017-08-01
期刊：
影响因子：
2.9
通讯作者：
Zhang, Jinghua
中科院分区：
文献类型：
--
作者：
Liu, Yankun;Han, Sugui;Zhang, Jinghua
据报道，MicroRNAs （miRs）与多种癌症的发生有关。然而，miRs在人卵巢癌化疗耐药中的作用在很大程度上仍未明确。在本研究中，细胞化疗结合细胞计数试剂盒-8检测表明，miR-20a在卵巢癌细胞化疗耐药中发挥重要作用。流式细胞术、细胞增殖实验和Transwell实验结果显示，OVCAR3/DDP细胞的增殖和迁移率较亲本细胞明显增加。Western blot分析结果表明，miR-20a激活的上皮-间质转化（epithelial-mesenchymal transition， EMT）促进了OVCAR3/DDP细胞的迁移。本研究强调了miR-20a在调节OVCAR3细胞的耐药特性以及通过激活EMT促进顺铂耐药细胞迁移中的重要性。因此，本研究的结果可能为逆转卵巢癌的化疗耐药和改善其治疗提供新的见解。
MicroRNAs (miRs) have been reported to be associated with the development of numerous types of cancer. However, the function of miRs in human ovarian carcinoma chemoresistance remains largely undefined. In the present study, cell chemotherapy combined with a Cell Counting Kit-8 assay demonstrated that miR-20a performed important roles in ovarian cancer cells chemoresistance. Flow cytometry, cellular proliferation assays and Transwell assays results revealed that the proliferation and migration rates of OVCAR3/DDP cells were increased in comparison with parental cells. Western blot analysis results suggested that epithelial-mesenchymal transition (EMT) activated by miR-20a contributed to OVCAR3/DDP cell migration. The present study highlighted the importance of miR-20a in regulating the chemoresistant properties of OVCAR3 cells and promoting cisplatin-resistant cell migration by activating EMT. The results of present study may therefore provide novel insights into reversing the chemoresistance of ovarian cancer and improving its treatment.