Cloning and characterization of the gene cluster encoding arthrofactin synthetase from Pseudomonas sp. MIS38.
Cloning and characterization of the gene cluster encoding arthrofactin synthetase from Pseudomonas sp. MIS38.
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DOI：
10.1016/j.chembiol.2003.09.004
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发表时间：
2003-09
影响因子：
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通讯作者：
Niran Roongsawang;K. Hase;M. Haruki;T. Imanaka;M. Morikawa;S. Kanaya
中科院分区：
文献类型：
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作者：
Niran Roongsawang;K. Hase;M. Haruki;T. Imanaka;M. Morikawa;S. Kanaya
节因子是假单胞菌产生的一种有效的环状脂肽型生物表面活性剂。MIS38.在这项工作中，一个artifactin合成酶基因簇（arf）跨越38.7 kb的克隆和鉴定。arfA、arfB和arfC三个基因分别编码ArfA、ArfB和ArfC，分别含有两个、四个和五个功能模块。每个模块带有缩合，腺苷酸化和巯基化结构域，像其他非核糖体肽合成酶。然而，与它们中的大多数不同，这11个模块中没有一个具有负责将氨基酸残基从L型转化为D型的差向异构化结构域。可能的L-和D-Leu腺苷酸化结构域仅特异性识别L-Leu。此外，两个硫酯酶结构域串联地位于ArfC的C末端。这些结果表明ArfA、ArfB和ArfC组装形成独特的结构。arfB的基因破坏损害了关节因子的产生，减少了群集活动，并增强了生物膜的形成。
Arthrofactin is a potent cyclic lipopeptide-type biosurfactant produced byPseudomonassp. MIS38. In this work, an arthrofactin synthetase gene cluster (arf) spanning 38.7 kb was cloned and characterized. Three genes termedarfA,arfB, andarfCencode ArfA, ArfB, and ArfC, containing two, four, and five functional modules, respectively. Each module bears condensation, adenylation, and thiolation domains, like other nonribosomal peptide synthetases. However, unlike most of them, none of the 11 modules possess the epimerization domain responsible for the conversion of amino acid residues from L to D form. Possible L- and D-Leu adenylation domains specifically recognized only L-Leu. Moreover, two thioesterase domains are tandemly located at the C-terminal end of ArfC. These results suggest that ArfA, ArfB, and ArfC assemble to form a unique structure. Gene disruption ofarfBimpaired arthrofactin production, reduced swarming activity, and enhanced biofilm formation.