Differences in lectin binding in squamous metaplasia induced by benzo(a)pyrene and vitamin A deficiency in hamster tracheal explants.
Differences in lectin binding in squamous metaplasia induced by benzo(a)pyrene and vitamin A deficiency in hamster tracheal explants.
复制标题
仓鼠气管外植体中苯并（a）芘和维生素 A 缺乏诱导的鳞状化生中凝集素结合的差异。
DOI：
10.1007/bf02630920
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Joiakim,AP
中科院分区：
文献类型：
--
作者：
Chopra,DP;Joiakim,AP
Epidermoid metaplasia in the hamster trachea can be produced by treatment with benzo(a)pyrene (BP) or vitamin A deficiency. To elucidate distinguishing features of the two types of lesions, lectin binding to tissue sections of tracheal explants exhibiting metaplastic lesions was assessed. In squamous metaplasia induced by vitamin A deficiency, Dolichos biflorus agglutinin (DBA), wheat germ agglutinin (WGA), and peanut agglutinin (PNA) showed faint (+) to moderate (++) binding in both basal and suprabasal cells; Concanavalin A (Con A) showed moderate binding (++) to suprabasal cells and no binding in basal cells. In the BP-induced lesions, PNA and WGA bound intensely (++++, +++, respectively) in basal cells and faintly (+) to moderately (++) in suprabasal cells. The staining seemed to be predominant at the periphery of the cells. Further, the intensity of PNA and WGA staining increased significantly after the neuraminidase treatment. DBA and Con A showed faint (+) to moderate (++) binding in the BP-induced metaplasia. The results show that in BP-induced metaplasia, cells in the basal region show preferential binding of PNA and WGA.
