Multicolor coding of cells with cationic peptide coated quantum dots
Multicolor coding of cells with cationic peptide coated quantum dots
复制标题
DOI：
10.1021/nl049295v
复制
发表时间：
2004-10-01
期刊：
影响因子：
10.8
通讯作者：
Waggoner, AS
中科院分区：
文献类型：
--
作者：
Lagerholm, BC;Wang, MM;Waggoner, AS
量子点是一类新型的荧光团，其更突出的特点是尺寸可调、窄的荧光发射带和宽的多色点重叠激发光谱。在这里，我们提出了一种有效的，多功能的，温和的方法，细胞内交付的量子点，很容易扩展到哺乳动物细胞的光编码。在该方法中，使用9个残基的生物素化的L-精氨酸肽来增强链霉亲和素缀合的量子点向哺乳动物细胞中的递送。
Quantum dots are a new class of fluorophores, whose more prominent characteristics include size-tunable, narrow, fluorescence emission bands and broad overlapping excitation spectra of multiple color dots. Here, we present an efficient, versatile, and gentle approach for intracellular delivery of quantum dots that is easily extended to multicolor optical coding of mammalian cells. In this method, a nine residue biotinylated L-arginine peptide is used to enhance delivery of streptavidin conjugated quantum dots into mammalian cells.