Surface plasmon resonance sensor for profenofos detection using molecularly imprinted thin film as recognition element
Surface plasmon resonance sensor for profenofos detection using molecularly imprinted thin film as recognition element
复制标题
使用分子印迹薄膜作为识别元件的用于丙溴磷检测的表面等离子体共振传感器
DOI：
10.1016/j.foodcont.2011.11.015
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发表时间：
2012-06-01
期刊：
影响因子：
6
通讯作者：
Gao, Zhixian
中科院分区：
文献类型：
--
作者：
Dong, Jianwei;Gao, Na;Gao, Zhixian
开发了一种使用分子印迹聚合物（MIP）超薄膜作为传感材料的表面等离子共振（SPR）传感器，用于检测自来水中的丙溴磷。 MIP 超薄膜通过表面引发的自由基聚合固定在金芯片上。金表面首先用11-巯基十一烷酸修饰，形成自组装单层（SAM）。为了轻松地将2,2-偶氮双(2-脒基丙烷)盐酸盐接枝到SAM上，使用1-(3-二甲基氨基丙基)-3-乙基碳二亚胺盐酸盐和N-羟基琥珀酰亚胺活化SAM的羧基。然后，以甲基丙烯酸为功能单体、三羟甲基丙烷三甲基丙烯酸酯为交联剂，通过热引发聚合合成了MIP薄膜。在此原理的基础上，研究了两种超薄膜制备方法并采用了最合适的技术。通过原子力显微镜对制备的超薄膜进行了表征。 MIP-SPR传感器用于检测自来水中添加0.001-0.1 μg mL(-1)杀虫剂的丙溴磷。该传感器表现出高灵敏度、选择性和良好的稳定性。该传感器的回收率和重复性(RSD,n=4)分别为89.81-100.99%和3.69-5.76%,而基于信噪比3(S/N=3)的检测限为3.6×10(-4)μg·mL(-1)。 (C) 2011 Elsevier Ltd. 保留所有权利。
A surface plasmon resonance (SPR) sensor that uses molecularly imprinted polymer (MIP) ultrathin film as sensing material was developed for detecting profenofos in tap water. The MIP ultrathin film was anchored on a gold chip by surface-initiated radical polymerization. The gold surface was first modified by 11-mercaptoundecanoic acid to form a self-assembled monolayer (SAM). To easily graft 2,2-azobis (2-amidinopropane) hydrochloride onto the SAM, the carboxyl of SAM was activated using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide. Then, the MIP film was synthesized by thermal-initiated polymerization using methacrylic acid as functional monomer and trimethylolpropane trimethacrylate as cross-linker. On the basis of this principle, two methods for ultrathin film preparation were studied and the most suitable technique was adopted. The prepared ultrathin film was characterized by atomic force microscopy. The MIP-SPR sensor was used to detect profenofos in tap water spiked with 0.001-0.1 mu g mL(-1) of the insecticide. The sensor showed high sensitivity, selectivity and good stability. The recoveries and repeatability (RSD, n = 4) of the sensor were 89.81-100.99% and 3.69-5.76%, respectively, while the detection limit based on a signal-to-noise ratio of 3 (S/N = 3) was 3.6 x 10(-4) mu g mL(-1). (C) 2011 Elsevier Ltd. All rights reserved.