Phosphorus metabolism and population dynamics in a biological phosphate-removal system with simultaneous anaerobic phosphate stripping.
Phosphorus metabolism and population dynamics in a biological phosphate-removal system with simultaneous anaerobic phosphate stripping.
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DOI：
10.1016/j.chemosphere.2014.10.018
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发表时间：
2014-12
期刊：
影响因子：
8.8
通讯作者：
Jinghua Lv;Lin-jiang Yuan;Xi Chen;Lun-Kuang Liu;Dacheng Luo
中科院分区：
文献类型：
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作者：
Jinghua Lv;Lin-jiang Yuan;Xi Chen;Lun-Kuang Liu;Dacheng Luo
在本研究中，磷的代谢和种群动态的变化进行了研究，通过同时在侧流化学剥离在乙酸进料序批式反应器。当生物量经受83 d的磷剥离时，聚磷菌（PAOs）合成的胞内聚磷酸盐（poly-P）逐渐减少。最初，系统的除磷效率从94.3%提高到96.9%。在此期间，预水解酶/羟基戊酸酯/羟基戊酸酯/PHA的化学计量比降低，糖原降解酶/羟基戊酸酯的化学计量比升高。结果表明，该菌的代谢由聚磷代谢向糖原代谢转变。相应地，PAO下降到不到1%的人口，糖原积累生物体增殖到近20%。PCR-DGGE结果还表明，随着聚磷含量的逐渐降低，微生物群落结构发生了显著变化。这些结果表明，胞内聚磷水平对磷去除系统的稳定是重要的。此外，侧流生物除磷系统厌氧段的磷回收并非一种稳定有效的途径。
In this study, the metabolism of phosphorus and changes in population dynamics were investigated via simultaneous chemical stripping in sidestream in an acetate-fed sequencing batch reactor. The synthesized intracellular polyphosphate (poly-P) by polyphosphate-accumulating organisms (PAOs) gradually decreased when the biomass was subjected to 83 d of P stripping. Initially, the P removal efficiency of the system improved from 94.3% to 96.9%. Thereafter, a relatively high level of P in effluent was observed, during which time the stoichiometric ratios of Prelease/HAcuptakedecreased, Glycogendegraded/HAcuptakeand poly-β-hydroxyvalerate/PHA increased. The results revealed that a metabolic shift from polyphosphate-accumulating metabolism to glycogen-accumulating metabolism. Correspondingly, PAOs declined to less than 1% of the population, glycogen-accumulating organisms proliferated to almost 20% instead. The results of PCR–DGGE also indicated that the microbial community structure considerably changed in response to the gradually decreasing poly-P content. These findings imply that intracellular poly-P level is important for the stable of P removal system. Furthermore, it suggests that it is not a stable and effective way for P recycling from anaerobic stage of the biological P removal system in sidestream.