Structure- and dose-absorption relationships of coffee polyphenols
Structure- and dose-absorption relationships of coffee polyphenols
复制标题
DOI：
10.1002/biof.1101
复制
发表时间：
2014-01-01
期刊：
影响因子：
6
通讯作者：
Richling, Elke
中科院分区：
文献类型：
--
作者：
Erk, Thomas;Hauser, Johanna;Richling, Elke
咖啡中的绿原酸（CGA）具有与人类健康相关的生物学效应。因此，关于它们在上胃肠道中的生物利用度的具体数据是高度感兴趣的，因为一些分子在这里被吸收，因此不被结肠微生物群代谢。到目前为止，还没有关于CGA的结构-吸收关系的数据发表，尽管这是西方饮食中消费最多的多酚类。为了解决这一差距，我们使用Ussing腔室模型（模拟粘膜及其腔/顶侧的模型）对猪空肠粘膜进行了体内吸收实验。主要的咖啡多酚，咖啡酰奎尼酸（CQA），阿魏酰奎尼酸（FQA），咖啡酸（CA），二咖啡酰奎尼酸（diCQA）和D-（-）-奎尼酸（QA），在单独的实验中孵育相当于肠腔生理可达到的浓度（0.2-3.5 mM）。采用HPLC-二极管阵列检测和HPLC-MS/MS进行鉴别和定量。此外，通过蛋白质印迹分析确定ABC-外排转运蛋白的存在。通过空肠猪粘膜吸收的初始施用的CGA的百分比按递增顺序为：diCQA，痕量; CQA，101%; CA，101.5%; FQA，102%;和QA，104%。在CGA亚组内未观察到差异。5-CQA的剂量吸收实验表明其为被动扩散（非饱和吸收和线性剂量-通量关系），其分泌受NaN 3影响，表明其为主动外排。首次在猪空肠粘膜中鉴定出ABC-外排转运蛋白MDR 1和MRP 2。我们的结论是，主动外排起着重要的作用，CGA的生物利用度，并进一步，CGA在空肠吸收的机制是由其物理化学性质。© 2013 BioFactors，40（1）：103-112，2014
Chlorogenic acids (CGAs) from coffee have biological effects related to human health. Thus, specific data on their bioavailability in the upper gastrointestinal tract are of high interest, since some molecules are absorbed here and so are not metabolized by colonic microflora. Up to now, no data on structure–absorption relationships for CGAs have been published, despite this being the most consumed group of polyphenols in the western diet. To address this gap, we performedex vivoabsorption experiments with pig jejunal mucosa using the Ussing chamber model (a model simulating the mucosa and its luminal/apical side). The main coffee polyphenols, caffeoylquinic acid (CQA), feruloylquinic acid (FQA), caffeic acid (CA), dicaffeoylquinic acid (diCQA), andD‐(−)‐quinic acid (QA), were incubated in individual experiments equivalent to gut lumen physiologically achievable concentrations (0.2–3.5 mM). Identification and quantification were performed with HPLC‐diode array detection and HPLC‐MS/MS. Additionally, the presence of ABC‐efflux transporters was determined by Western blot analysis. The percentages of initially applied CGAs that were absorbed through the jejunal pig mucosa were, in increasing order: diCQA, trace; CQA, ≈1%; CA, ≈1.5%; FQA, ≈2%; and QA, ≈4%. No differences were observed within the CGA subgroups. Dose–absorption experiments with 5‐CQA suggested a passive diffusion (nonsaturable absorption and a linear dose–flux relationship) and its secretion was affected by NaN3, indicating an active efflux. The ABC‐efflux transporters MDR 1 and MRP 2 were identified in pig jejunal mucosa for the first time. We conclude that active efflux plays a significant role in CGA bioavailability and, further, that the mechanism of CGA absorption in the jejunum is governed by their physicochemical properties. © 2013 BioFactors, 40(1):103–112, 2014