Left ventricular pressure-volume relationship in conscious mice
Left ventricular pressure-volume relationship in conscious mice
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DOI：
10.1152/ajpheart.00704.2006
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发表时间：
2007-01-01
影响因子：
4.8
通讯作者：
Grossman, William
中科院分区：
文献类型：
--
作者：
Joho, Shuji;Ishizaka, Shinji;Grossman, William
随着转基因模型的出现，小鼠已成为遗传血流动力学研究日益重要的对象。最近，我们开发了一种技术，用于测量植入左心室聚乙烯管的清醒小鼠的左心室 (LV) 压力。我们通过评估左心室压力-容积关系扩展了我们的新方法，并在本研究中检验了该方法的可行性。我们研究了 17 只雄性小鼠（年龄，11 - 20 周），将电导导管通过聚乙烯管插入左心室。通过使用植入圈套器进行下腔静脉闭塞，评估源自压力-容量关系的与负荷无关的收缩性参数[收缩末期压力-容量关系的斜率（E-es）、左心室压力最大一阶导数的斜率（dP/dt(max)）-舒张末期容量（EDV）关系以及预负荷-可复张每搏功（PRSW）]。通过该技术在不同的两天评估的左心室功能显示参数非常相似，表明可重复性。对 Ees 进行了线性和非线性回归分析。异丙肾上腺素 (Ees，13.1 +/- 6.6 至 20.8 +/- 8.7 mmHg/μl；dP/dt(max)- EDV，496 +/- 139 至 825 +/- 178 mmHg (.) s (-1 .) mu l(-1)；PRSW，110 +/- 23 至127 +/- 21 mmHg)，被阿替洛尔抑制（E-es，14.5 +/- 6.1 至 4.6 +/- 2.0 mmHg/μl；dP/dt(max)- EDV，543 +/- 188 至 185 +/- 94 mmHg (.) s(-1 .) mu l (-1)；PRSW，117 +/- 20 至 70 +/- 15 mmHg) 和异氟醚（E-es，12.3 +/- 6.0 至 5.7 +/- 2.1 mmHg/μl；dP/dt(max)- EDV，528 +/- 172 至 164 +/- 68 mmHg/s (.)μl；和 PRSW，124 +/- 19 至 48 +/- 10 mmHg），显着。总之，这是对清醒小鼠左心室压力-容量关系的首次描述。这些发现表明，这种方法可以检测意识状态下收缩力的变化，从而可以随着时间的推移对压力-容量衍生的心功能指数进行连续评估，而无需麻醉或重复手术。
With the availability of transgenic models, the mouse has become an increasingly important subject for genetic- hemodynamic studies. Recently, we developed a technique to measure left ventricular ( LV) pressure in conscious mice with an implanted LV polyethylene tube. We extended our new method by evaluating the LV pressure-volume relationship and examined the feasibility of this method in this study. We studied 17 male mice ( age, 11 - 20 wk) with a conductance catheter inserted into the LV through the polyethylene tube. Load- independent parameters of contractility derived from pressure- volume relationship [ slope of the end- systolic pressure- volume relationship ( E-es), slope of the maximum first derivative of LV pressure ( dP/dt(max))- end- diastolic volume ( EDV) relation, and preload- recruitable stroke work (PRSW)] were evaluated by inferior vena caval occlusion with an implanted snare. LV function assessed by this technique on two different days showed that the parameters were very similar, indicating reproducibility. Both linear and nonlinear regression analyses were performed for Ees. Contractility was enhanced by isoproterenol ( Ees, 13.1 +/- 6.6 to 20.8 +/- 8.7 mmHg/ mu l; dP/ dt(max)- EDV, 496 +/- 139 to 825 +/- 178 mmHg (.) s (-1 .) mu l(-1); and PRSW, 110 +/- 23 to 127 +/- 21 mmHg), depressed by atenolol ( E-es, 14.5 +/- 6.1 to 4.6 +/- 2.0 mmHg/mu l; dP/dt(max)- EDV, 543 +/- 188 to 185 +/- 94 mmHg (.) s(-1 .) mu l (-1); and PRSW, 117 +/- 20 to 70 +/- 15 mmHg) and isoflurane ( E-es, 12.3 +/- 6.0 to 5.7 +/- 2.1 mmHg/mu l; dP/ dt(max)- EDV, 528 +/- 172 to 164 +/- 68 mmHg/ s (.) mu l; and PRSW, 124 +/- 19 to 48 +/- 10 mmHg), significantly. In conclusion, this is the first description of the LV pressure- volume relationship in conscious mice. These findings suggest that this method is feasible to detect changes of contractility in the conscious state, allowing serial assessment of pressure- volume- derived cardiac function indexes over time without anesthesia or repeated surgery.