cis-Acting Elements Responsible for Low-Temperature-Inducible Expression of the Gene Coding for the Thermolabile Isocitrate Dehydrogenase Isozyme of a Psychrophilic Bacterium, Vibrio sp. Strain ABE-1
cis-Acting Elements Responsible for Low-Temperature-Inducible Expression of the Gene Coding for the Thermolabile Isocitrate Dehydrogenase Isozyme of a Psychrophilic Bacterium, Vibrio sp. Strain ABE-1
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顺式作用元件负责编码嗜冷细菌弧菌的不耐热异柠檬酸脱氢酶同工酶的基因的低温诱导表达。
DOI：
10.1128/jb.181.8.2602-2611.1999
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发表时间：
1999
影响因子：
3.2
通讯作者：
N. Fukunaga
中科院分区：
文献类型：
--
作者：
T. Sahara;Masahiro Suzuki;J. Tsuruha;Y. Takada;N. Fukunaga
低温诱导icdII基因编码嗜冷细菌（弧菌属菌株ABE-1）的不耐热异柠檬酸脱氢酶，其转录控制被发现部分由位于icdII转录起始位点上游-560至-526核苷酸位置的转录沉默子介导。沉默子的缺失导致该基因在低温（15°C）下的表达水平增加20倍，但在高温（37°C）下则没有。此外，位于-35区域上游2个碱基的CCAAT序列被发现是该基因低温诱导表达所必需的。通过删除该序列，该基因的低温依赖性表达被完全消除。通过使用含有icd II启动子区域和无启动子icd I开放阅读框的融合基因证实icd II启动子控制其它基因表达的能力，所述融合基因编码弧菌属菌株ABE-1的非冷诱导型异柠檬酸脱氢酶同工酶。携带icdII的大肠杆菌转化体获得了在低温下快速生长的能力。
ABSTRACT Transcriptional control of the low-temperature-inducibleicdII gene, encoding the thermolabile isocitrate dehydrogenase of a psychrophilic bacterium, Vibrio sp. strain ABE-1, was found to be mediated in part by a transcriptional silencer locating at nucleotide positions −560 to −526 upstream from the transcription start site of icdII. Deletion of the silencer resulted in a 20-fold-increased level of expression of the gene at low temperature (15°C) but not at high temperature (37°C). In addition, a CCAAT sequence located 2 bases upstream of the −35 region was found to be essential for the low-temperature-inducible expression of the gene. By deletion of this sequence, low-temperature-dependent expression of the gene was completely abolished. The ability of the icdII promoter to control the expression of other genes was confirmed by using a fusion gene containing the icdII promoter region and the promoterlessicdI open reading frame, which encodes the non-cold-inducible isocitrate dehydrogenase isozyme ofVibrio sp. strain ABE-1. Escherichia colitransformants harboring icdII acquired an ability to grow rapidly at low temperature.