Facile and Rapid Detection of Microalbuminuria by Antibody-Functionalized Gold Nanorods
Facile and Rapid Detection of Microalbuminuria by Antibody-Functionalized Gold Nanorods
复制标题
DOI：
10.1007/s11468-022-01606-x
复制
发表时间：
2022-03-08
期刊：
影响因子：
3
通讯作者：
H. Sajedi, Reza
中科院分区：
文献类型：
--
作者：
Mirjalili, Seyedsina;Tohidi Moghadam, Tahereh;H. Sajedi, Reza
尿中人血清白蛋白（HSA）的任何过量或缺乏都应被视为肾脏或心血管疾病的警告。本报告提出了一种基于表面等离子体共振特性和抗体功能化金纳米棒（GNRs）追踪局部环境变化的敏感性的快速直接检测HSA的方法。还研究了电位参数（如pH值、抗体浓度和阻断剂）的作用，以优化传感器的性能。纳米生物传感器在30 ~ 300 μ g.mL（-1）浓度范围内检测HSA，检出限为1 μ g.mL(-1)，对HSA具有较高的选择性。该纳米生物传感器在检测微量白蛋白尿或大量白蛋白尿所需的参考范围内具有检测限。所开发的纳米生物传感器还显示了在尿液样本中追踪HSA的可能性，准确率为95-100%。本研究介绍了基于金纳米棒的传感器的潜在应用，作为传统方法的有希望的替代品，以新一代简便、快速和“瓶中实验室”的纳米诊断技术的形式出现。
Any excess or lack of human serum albumin (HSA) in urine should be considered a warning for renal or cardiovascular diseases. This report proposes a rapid and straightforward method for detecting HSA based on the surface plasmon resonance characteristics and sensitivity of antibody-functionalized gold nanorods (GNRs) to trace changes in the local environment. The role of potential parameters (e.g., pH, the concentration of antibody, and the blocking agent) was also studied to optimize the sensor performance. The nanobiosensor determined HSA in the 30-300 mu g.mL(-1) concentration range with a detection limit of 1 mu g.mL(-1) and exhibited high selectivity towards HSA. This nanobiosensor has a detection limit within the reference range required to detect microalbuminuria or macroalbuminuria. The developed nanobiosensor also showed the possibility of tracing HSA in urine samples with 95-100% accuracy. This investigation introduces a potential application of gold nanorod-based sensors as promising alternatives for conventional methods in the form of a new generation of facile, rapid, and "lab-in-a-vial" nanodiagnostic technique.