Phosphatase of Chlamydomonas reinhardi: biochemical and cytochemical approach with specific mutants
Phosphatase of Chlamydomonas reinhardi: biochemical and cytochemical approach with specific mutants
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莱茵衣藻磷酸酶：特定突变体的生化和细胞化学方法
DOI：
10.1128/jb.126.2.937-950.1976
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发表时间：
1976
影响因子：
3.2
通讯作者：
R. Deltour
中科院分区：
文献类型：
--
作者：
R. Matagne;R. Loppes;R. Deltour
单细胞藻类莱茵衣藻产生两种组成型酸性磷酸酶和三种抑制性磷酸酶（一种中性磷酸酶和两种碱性磷酸酶），它们可以利用磷酸萘酯作为底物。利用特异性突变体抑制性磷酸酶研究了这些酶的生化特性和细胞化学定位。这两种组成型磷酸酶表现出相似的最佳pH值（约5.0）和Km值（2 × 10（-3）至3.3 × 10(-3) M），但它们的热敏性和对甘油磷酸盐的亲和力不同。
The unicellular alga Chlamydomonas reinhardi produces two constitutive acid phosphatases and three depressible phosphatases (a neutral and two alkaline ones) that can utilize napthyl phosphate as a substrate. Specific mutants depressible phosphatase were used to investigate biochemical properties and the cytochemical localization of these enzymes. The two constitutive phosphatases show similar pH optima (about 5.0) and Km values (2 x 10(-3) to 3.3 x 10(-3) M) but differ in their heat sensitivity and affinity for glycerophosphate.