Generation of a double insulin and somatostatin reporter line, SCSe001-A-3, for the advancement of stem cell-derived pancreatic islets.
Generation of a double insulin and somatostatin reporter line, SCSe001-A-3, for the advancement of stem cell-derived pancreatic islets.
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DOI：
10.1016/j.scr.2020.102112
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发表时间：
2020-12-08
影响因子：
1.2
通讯作者：
Gadue P
中科院分区：
文献类型：
--
作者：
Leavens KF;Liao CM;Gagne AL;Kishore S;Cardenas-Diaz FL;French DL;Gadue P
在过去的十年里，在通过定向分化从人类干细胞发展成胰腺β细胞方面取得了显著的进展，从而可以对β细胞的发育、功能和疾病进行建模。然而，体外模型和未来的治疗应用将需要使用干细胞来源的具有多种单激素内分泌细胞类型的胰岛，包括α，β和δ细胞。我们利用已报道的Mel1 InsGFP/w人胚胎干细胞(HESC)系，利用CRISPR/Cas9，在内源性生长抑素启动子的控制下，敲入红色荧光蛋白(RFP)，获得了胰岛素和生长抑素双重报告基因hESC。
Remarkable strides have been made over the past decade on the development of pancreatic β-cells from human stem cells through directed differentiation, allowing for modeling of β-cell development, function and disease. However, in vitro models and future therapeutic applications will require the use of stem cell-derived islets with multiple monohormonal endocrine cells types, including α, β, and δ cells. Using the previously reported Mel1 InsGFP/w human embryonic stem cell (hESC) line, we have knocked-in Red Fluorescence Protein (RFP) under the control of the endogenous somatostatin promoter using CRISPR/Cas9, generating a dual insulin and somatostatin reporter hESC line.
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影响因子：
5.9
作者：
Tiyaboonchai A;Cardenas-Diaz FL;Ying L;Maguire JA;Sim X;Jobaliya C;Gagne AL;Kishore S;Stanescu DE;Hughes N;De Leon DD;French DL;Gadue P
通讯作者：
Gadue P
影响因子：
8.2
作者：
Micallef, S. J.;Li, X.;Stanley, E. G.
通讯作者：
Stanley, E. G.
影响因子：
23.9
作者：
Cardenas-Diaz, Fabian L.;Osorio-Quintero, Catherine;Gadue, Paul
通讯作者：
Gadue, Paul
影响因子：
1.2
作者：
Maguire, Jean Ann;Gagne, Alyssa L.;French, Deborah L.
通讯作者：
French, Deborah L.