Linkage map organization of expressed sequence tags and sequence tagged sites in the mosquito, Aedes aegypti
Linkage map organization of expressed sequence tags and sequence tagged sites in the mosquito, Aedes aegypti
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DOI：
10.1046/j.1365-2583.2002.00347.x
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发表时间：
2002-08-01
影响因子：
2.6
通讯作者：
Morlais, I
中科院分区：
文献类型：
--
作者：
Severson, DW;Meece, JK;Morlais, I
利用限制性片段长度多态性（RFLP）、单核苷酸多态性（SNP）和单链构象多态性（SSCP）标记构建了埃及伊蚊（Aedesaegypti）的复合遗传连锁图谱。该图谱由分布在205 cM的146个标记位点组成，并包括几个形态突变标记位点。大多数遗传标记来自随机cDNA或Ale。埃及人已知功能的基因。许多标记来源于随机基因组DNA，包括几个克隆的RAPD-PCR片段，以及几个来自果蝇的cDNA。大部分随机cDNA（80.2%）与已知基因具有较高的BLASTX序列同源性，其中大部分与D.黑腹菌获得所有标记的序列数据将促进其用于高通量SNP标记分析的持续开发，并且还为预期的基因组测序工作提供额外的物理锚点。
A composite genetic linkage map for the yellow fever mosquito Aedes aegypti was constructed based on restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and single strand conformation polymorphism (SSCP) markers. The map consists of 146 marker loci distributed across 205 cM, and includes several morphological mutant marker loci. Most of the genetic markers are derived from random cDNAs or Ale. aegypti genes of known function. A number of markers are derived from random genomic DNAs, including several cloned RAPD-PCR fragments, and also several cDNAs from Drosophila melanogaster. Most of the random cDNAs (80.2%) have high BLASTX sequence identities to known genes, with the majority of matches to genes from D. melanogaster. Access to sequence data for all markers will facilitate their continued development for use in high-throughput SNP marker analyses and also provides additional physical anchor points for an anticipated genome sequencing effort.