Aryl alcohol oxidases from the white-rot basidiomycete Pleurotus ostreatus
Aryl alcohol oxidases from the white-rot basidiomycete Pleurotus ostreatus
复制标题
来自白腐担子菌平菇的芳基醇氧化酶
DOI：
10.1007/s102670200057
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发表时间：
2002
期刊：
影响因子：
1.4
通讯作者：
H. Yanase
中科院分区：
文献类型：
--
作者：
K. Okamoto;H. Yanase
采用多层色谱法从一株白腐菌平菇培养滤液中纯化了3种芳醇氧化酶（AAOs; EC 1.1.3.7） I、II和III。纯化的AAOs I、II和III在凝胶过滤层析和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上的分子量分别为70 kDa和72 kDa。它们的最适温度为40℃，但最适ph值略有差异。aaaos I、II和III的n端氨基酸序列分别为ala - asp - lys - asp - tyr - il - val - val - gly - ala，与pleurotus eryngii（80%同源）和pleurotus ostreatusFlorida（60%同源）具有显著的相似性。
Three aryl alcohol oxidases (AAOs; EC 1.1.3.7) I, II, and III from the culture filtrate of a strain of white-rot fungusPleurotus ostreatuswere purified by multistep chromatography. Each of the purified AAOs I, II, and III had the same molecular masses of 70 kDa and 72 kDa on gel filtration chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, respectively. Their optimum temperature was 40°C, but their optimum pHs differed slightly. The N-terminal amino acid sequence of AAOs I, II, and III was determined to be Ala-Asp-Lys-Asp-Tyr-Ile-Val-Val-Gly-Ala, which showed significant similarity to those ofPleurotus eryngii(80% identity) andPleurotus ostreatusFlorida (60% identity).