Anti-human olig2 antibody as a useful immunohistochemical marker of normal oligodendrocytes and gliomas
Anti-human olig2 antibody as a useful immunohistochemical marker of normal oligodendrocytes and gliomas
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DOI：
10.1016/s0002-9440(10)63730-3
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发表时间：
2004-05-01
影响因子：
6
通讯作者：
Nakazato, Y
中科院分区：
文献类型：
--
作者：
Yokoo, H;Nobusawa, S;Nakazato, Y
Olig2是最近发现的一种转录因子，参与了少突胶质细胞谱系中细胞的表型定义。Olig2转录物在人少突胶质细胞肿瘤中的表达已被证实，但其蛋白表达尚未得到广泛研究。我们制备了一种针对人Olig2的多克隆抗体，并对其进行免疫组织化学分析。通过Western blotting，该抗体描述了一个不同的预测分子量带，并且不与人Olig1交叉反应。在正常人脑组织中，束间、血管周围和神经元周围少突胶质细胞的细胞核被抗体清晰地标记。同样，少突胶质肿瘤的细胞核也被标记。染色强度与组织学分级无明显相关性。肿瘤内星形细胞成分一般较少染色或未染色。星形胶质细胞肿瘤Olig2抗血清也有较低程度的阳性，统计学分析证实了少突胶质细胞肿瘤与星形细胞肿瘤的差异。Olig2和胶质原纤维酸性蛋白以互斥的方式表达，Olig2的表达与细胞周期相关。中枢神经细胞瘤和神经鞘瘤均未染色。我们的抗体在石蜡切片上可识别正常少突胶质细胞，并可用于某些脑肿瘤的诊断。
Olig2 is a recently identified transcription factor involved in the phenotype definition of cells in the oligodendroglial lineage. The expression of Olig2 transcript has been demonstrated in human oligodendroglial tumors, although the protein expression has not been studied extensively. We developed a polyclonal antibody to human Olig2 and analyzed it immunohistochemically. The antibody depicted a single distinct band of predicted molecular weight by Western blotting, and did not cross-react with human Olig1. in normal human brain tissue, the nuclei of oligodendrocytes of interfascicular, perivascular, and perineuronal disposition were clearly labeled by the antibody. Similarly, the nuclei of oligodendroglial tumors were labeled. There was no apparent correlation between the staining intensity and histological grade. Astrocytic components within the tumors were generally less or not stained. Astrocytic tumors were also positive with the Olig2 antiserum to a lesser extent, and the difference between oligodendroglial and astrocytic tumors was demonstrated by a statistical analysis. Olig2 and glial fibrillary acidic protein were expressed in a mutually exclusive manner, and Olig2 expression was cell-cycle related. Neither central neurocytoma nor schwannoma cases were stained. Our antibody was demonstrated to be useful in recognizing normal oligodendrocytes on paraffin sections, and applicable in diagnosis of some brain tumors.