Cytopathogenicity of cytomegalovirus to human ecto- and endocervical epithelial cells in vitro.
Cytopathogenicity of cytomegalovirus to human ecto- and endocervical epithelial cells in vitro.
复制标题
巨细胞病毒对人宫颈外和宫颈内上皮细胞的体外细胞致病性。
作者：
E. Vesterinen;P. Leinikki;E. Saksela
研究了巨细胞病毒对人子宫内膜和外宫颈细胞培养的影响。获得了纯上皮培养物，其形态根据来源而不同。子宫颈外段细胞生长在镶嵌状的规则上皮模式，而子宫颈内培养物具有较差的细胞间凝聚力，不规则的多边形细胞形式和弯曲的胞质突起。偶尔成纤维细胞集落生长在三个32宫颈外培养和宫颈内培养。在后者中，没有细胞与成纤维细胞特异性表面抗原可以看到。子宫颈外膜细胞对巨细胞病毒感染有抵抗力;只有在20个培养物中的3个中可以检测到个别改变的细胞。相比之下，宫颈培养支持巨细胞病毒的生长，最多30%的接种菌落显示细胞学改变以及病毒特异性免疫荧光。描述了各种类型的改变的细胞，并得出结论，宫颈内上皮细胞可能是宫颈分泌物中常见的人巨细胞病毒的复制部位。
The effect of cytomegalovirus on cell cultures initiated from human uterine endo- and ectocervix was studied. Pure epithelial cultures were obtained which differed in morphology depending on the source. The ectocervical cells grew in mosaic-like regular epithelial patterns, whereas endocervical cultures had a poorer intercellular cohesion, irregular polygonal cell form and curved cytoplasmic processes. Occasional fibroblastic colonies grew in three out of 32 ectocervical cultures and in none of the endocervical cultures. In the latter no cells with fibroblast-specific surface antigens could be seen. Ectocervical cells were resistant to cytomegalovirus infection; only in three out of 20 cultures could individual altered cells be detected. In contrast, endocervical cultures supported the growth of cytomegalovirus and at best 30 per cent of the explanted colonies showed cytologic alterations as well as virus-specific immunofluorescence. The various types of altered cells are described and it si concluded that endocervical epithelial cells may be the site of replication of human cytomegalovirus so commonly found in cervical secretions.