The comet assay in clinical practice
The comet assay in clinical practice
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DOI：
10.1080/028418699432527
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发表时间：
1999-01-01
期刊：
影响因子：
3.1
通讯作者：
Trotter, T
中科院分区：
文献类型：
--
作者：
Olive, PL;Durand, RE;Trotter, T
彗星试验是一种单细胞凝胶电泳技术，可以测量单个细胞的DNA损伤。因为辐射对氧气充足的细胞造成的DNA损伤是低氧细胞的3-4倍。这项检测可以量化在许多固体谣言中发现的耐辐射低氧细胞的比例。本文总结了73例接受姑息性放射治疗的可接近转移瘤的结果。低氧分数从0.0到0.67不等，平均值为0.15；这些晚期谣言中有62%显示出低氧分数>；0.05。对33个肿瘤的两次连续抽吸进行比较，斜率为0.92(r(2)=0.88)，表明一次抽吸通常代表肿瘤。然而，一个限制是，在常规剂量为2GY的临床样本中，无法测量到低氧部分。
Thr comet assay is a single-cell gel electrophoresis technique that measures DNA damage in individual cells. Since radiation produces 3-4 times more DNA damage in well-oxygenated cells compared with hypoxic cells. this assay can quantify the fraction of radiation-resistant hypoxic cells found in many solid rumours. This paper summarizes our results with 73 accessible metastatic tumours irradiated with palliative intent. Hypoxic fractions ranged from 0.0 to 0.67 with a mean of 0.15; 62% of these advanced rumours showed a hypoxic fraction > 0.05. Comparisons between two sequential aspirates in 33 tumours gave a slope of 0.92 (r(2) = 0.88), suggesting that a single aspirate is generally representative of the tumour. A limitation, however, is that the hypoxic fraction could not be measured in clinical samples given a conventional dose of 2 Gy.