Neisseria gonorrhoeae Sequence Typing for Antimicrobial Resistance, a Novel Antimicrobial Resistance Multilocus Typing Scheme for Tracking Global Dissemination of N-gonorrhoeae Strains
Neisseria gonorrhoeae Sequence Typing for Antimicrobial Resistance, a Novel Antimicrobial Resistance Multilocus Typing Scheme for Tracking Global Dissemination of N-gonorrhoeae Strains
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DOI：
10.1128/jcm.00100-17
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发表时间：
2017-05-01
影响因子：
9.4
通讯作者：
Martin, I.
中科院分区：
文献类型：
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作者：
Demczuk, W.;Sidhu, S.;Martin, I.
开发了一种基于淋病奈瑟菌抗菌素耐药性决定簇的、基于网络的用户友好型序列分型工具，并可公开访问（https：ngstar.canada.ca）。了N.淋病耐药性序列分型（NG-STAR）分子分型方案使用与β-内酰胺类抗生素、大环内酯类或氟喹诺酮类耐药性相关的7个基因（佩纳、mtrR、porB、ponA、gyrA、parC和23 S rRNA）的DNA序列。NG-STAR使用完整的佩纳序列，将I型至XXXVIII型佩纳的历史命名法与新的核苷酸序列名称相结合;完整的mtrR序列及其启动子区的一部分; ponA、porB、gyrA和parC的部分;以及23 S rRNA序列。NG-STAR将768个分离株分为139个序列类型（ST）（n = 660），其中29个克隆复合体（CC）具有最大的单位点变异，76个NG-STAR ST（n = 109）被鉴定为不相关的单基因型。NG-STAR的Simpson多样性指数值较高，为96.5%（95%置信区间[CI] = 0.959至0.969）。最常见的ST为NG-STAR ST-90（n = 100; 13.0%）、ST-42和ST-91（n = 45; 5.9%）、ST-64（n = 44; 5.72%）和ST-139（n = 42; 5.5%）。阿奇霉素敏感性降低与NG-STAR ST-58、ST-61、ST-64、ST-79、ST-91和ST-139相关（n = 156; 92.3%）;头孢菌素类药物敏感性降低与NG-STAR ST-90、ST-91和ST-97相关（n = 162; 94.2%）;环丙沙星耐药性与NG-STAR ST-26、ST-90、ST-91、ST-97、ST-150和ST-158相关（n = 196; 98.0%）。NG-STAR ST 42、ST-43、ST-63、ST-81和ST-160的所有分离株（n = 106）对所有4种抗菌剂均敏感。通过一个国际可用的数据库，将与抗菌素耐药决定因素相关的命名标准化，将有助于监测抗菌素耐药N.淋病菌株。
A curated Web-based user-friendly sequence typing tool based on antimicrobial resistance determinants in Neisseria gonorrhoeae was developed and is publicly accessible (https://ngstar.canada.ca). The N. gonorrhoeae Sequence Typing for Antimicrobial Resistance (NG-STAR) molecular typing scheme uses the DNA sequences of 7 genes (penA, mtrR, porB, ponA, gyrA, parC, and 23S rRNA) associated with resistance to beta-lactam antimicrobials, macrolides, or fluoroquinolones. NG-STAR uses the entire penA sequence, combining the historical nomenclature for penA types I to XXXVIII with novel nucleotide sequence designations; the full mtrR sequence and a portion of its promoter region; portions of ponA, porB, gyrA, and parC; and 23S rRNA sequences. NG-STAR grouped 768 isolates into 139 sequence types (STs) (n = 660) consisting of 29 clonal complexes (CCs) having a maximum of a single-locus variation, and 76 NG-STAR STs (n = 109) were identified as unrelated singletons. NG-STAR had a high Simpson's diversity index value of 96.5% (95% confidence interval [CI] = 0.959 to 0.969). The most common STs were NG-STAR ST-90 (n = 100; 13.0%), ST-42 and ST-91 (n = 45; 5.9%), ST-64 (n = 44; 5.72%), and ST-139 (n = 42; 5.5%). Decreased susceptibility to azithromycin was associated with NG-STAR ST-58, ST-61, ST-64, ST-79, ST-91, and ST-139 (n = 156; 92.3%); decreased susceptibility to cephalosporins was associated with NG-STAR ST-90, ST-91, and ST-97 (n = 162; 94.2%); and ciprofloxacin resistance was associated with NG-STAR ST-26, ST-90, ST-91, ST-97, ST-150, and ST-158 (n = 196; 98.0%). All isolates of NG-STAR ST42, ST-43, ST-63, ST-81, and ST-160 (n = 106) were susceptible to all four antimicrobials. The standardization of nomenclature associated with antimicrobial resistance determinants through an internationally available database will facilitate the monitoring of the global dissemination of antimicrobial-resistant N. gonorrhoeae strains.