Imaging dendritic spines: molecular organization and signaling for plasticity
Imaging dendritic spines: molecular organization and signaling for plasticity
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DOI：
10.1016/j.conb.2020.08.006
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发表时间：
2021-04-01
影响因子：
5.7
通讯作者：
Nishiyama, Jun
中科院分区：
文献类型：
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作者：
Suratkal, Swathi Shivaram;Yen, Yu-Hsin;Nishiyama, Jun
树突棘的结构可塑性被认为对于各种形式的突触可塑性以及最终的学习和记忆至关重要。该过程由信号通路介导，促进肌动蛋白细胞骨架和突触亚结构的重组，进而引起树突棘的结构和功能变化。光学技术的最新进展已经开始揭示脊柱内部发生的精细分子结构和动态信号传导，为脊柱的分子调控提供了重要的见解。在这里，我们重点介绍最近的研究，以高时空分辨率解析脊柱肌动蛋白细胞骨架和可塑性背后的分子机制。此外，我们讨论了基于基因组编辑的新方法来对树突棘的分子结构和可塑性进行成像。
The structural plasticity of dendritic spines is considered to be essential for various forms of synaptic plasticity and, ultimately, learning and memory. The process is mediated by signaling pathways that promote the reorganization of the actin cytoskeleton and subsynaptic structures, which in turn cause structural and functional changes in dendritic spines. Recent advances in optical technologies have started to reveal the fine molecular structures and dynamic signaling occurring inside spines, providing significant insights into the molecular regulation of spines. Here, we highlight recent studies to resolve the molecular mechanisms underlying the spine actin cytoskeleton and plasticity with high spatiotemporal resolution. Moreover, we discuss new genome editing-based approaches in imaging the molecular structure and plasticity of dendritic spines.