ULTRA-HIGH FIELD NMR SPECTROSCOPIC STUDIES ON HUMAN SEMINAL FLUID, SEMINAL-VESICLE AND PROSTATIC SECRETIONS
ULTRA-HIGH FIELD NMR SPECTROSCOPIC STUDIES ON HUMAN SEMINAL FLUID, SEMINAL-VESICLE AND PROSTATIC SECRETIONS
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DOI：
10.1016/0731-7085(94)80004-9
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发表时间：
1994-01-01
影响因子：
3.4
通讯作者：
NICHOLSON, JK
中科院分区：
文献类型：
--
作者：
LYNCH, MJ;MASTERS, J;NICHOLSON, JK
超高场H-1-NMR光谱方法已被用于分析精液及其成分分泌物，前列腺和精囊液从正常人科目和那些与血管发育不全和非梗阻性不孕症的组成。整个精液的H-1-NMR谱是极其复杂的，并且即使在600或750 MHz H-1共振频率下测量时，许多共振在单脉冲谱中也广泛重叠。在600或750 MHz下应用2-D H-1-NMR方法（包括J-解析和各种H-1同源相关和H-1-C-13异源相关技术）的组合，以广泛地分配来自精液有机组分的信号。前列腺液（PF）的代谢产物比整个精液的代谢产物复杂得多，可以使用500 MHz的H-1-NMR光谱进行完全分析。前列腺液的H-1-NMR谱主要由柠檬酸盐、精胺和肌醇的信号支配，而精囊液（SVF）的谱显示出来自复杂肽混合物的广泛重叠信号以及甘油磷酸胆碱（GPC）和乳酸盐的强信号。全精液是PF和SVF成分以及混合后由于PF酶对SVF的作用而出现的其他物质的组合，例如肽酶活性导致肽快速裂解为氨基酸，GPC水解为胆碱、甘油和无机磷酸盐。它还表明，输精管发育不全导致高度特征性的异常代谢产物在精液中，可以很容易地观察到在单脉冲500和600 MHz的H-1-NMR光谱。测量精液中柠檬酸盐与胆碱或精胺与胆碱的摩尔比，均显示血管发育不全患者与非梗阻性不孕患者之间存在2个数量级的差异（两种比例均较大）。这项工作给出了高场H-1-NMR光谱在男性生殖道的分泌功能的调查和评估和不育男性受试者的评价的潜力的指示。
Ultra high field H-1-NMR spectroscopic methods have been used to analyse the composition of seminal fluid and its component secretions, prostatic and seminal vesicle fluids from normal human subjects and those with vasal aplasia and non-obstructive infertility. The H-1-NMR spectrum of whole seminal fluid is extremely complex and many resonances are extensively overlapped in single pulse spectra even when measured at 600 or 750 MHz H-1 resonance frequency. A combination of 2-D H-1-NMR methods (including J-Resolved and various H-1 homonuclear correlation and H-1-C-13 heteronuclear correlation techniques) were applied at 600 or 750 MHz in order to extensively assign the signals from the organic components of seminal fluid. Prostatic fluid (PF) gives a much less complex metabolite profile than whole seminal fluid and can be completely analysed using 500 MHz H-1-NMR spectroscopy. The H-1-NMR spectra of prostatic fluid are dominated by signals from citrate, spermine and myo-inositol, whereas the spectra of seminal vesicle fluid (SVF) show extensively overlapped signals from complex peptide mixtures together with strong signals for glycerophosphocholine (GPC) and lactate. Whole seminal fluid is a combination of the PF and SVF constituents together with further substances that appear after mixing due to the operation of PF enzymes on SVF, e.g. peptidase activity causes rapid cleavage of peptides to amino acids and GPC is hydrolysed to choline, glycerol and inorganic phosphate. It is also shown that vasal aplasia leads to highly characteristic abnormal metabolite profiles in seminal fluid that can be readily observed in single-pulse 500 and 600 MHz H-1-NMR spectra. Measurement of the molar citrate to choline, or spermine to choline ratios in seminal fluid both show differences of 2 orders of magnitude between vasal aplasia (greater for both ratios) and non-obstructed infertile patients. This work gives an indication of the potential of high field H-1-NMR spectroscopy in the investigation and assessment of the secretory functions of the male genital tract and the evaluation of the infertile male subject.