RNAi of the circadian clock gene period disrupts the circadian rhythm but not the circatidal rhythm in the mangrove cricket
RNAi of the circadian clock gene period disrupts the circadian rhythm but not the circatidal rhythm in the mangrove cricket
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DOI：
10.1098/rsbl.2012.0079
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发表时间：
2012-08-23
期刊：
影响因子：
3.3
通讯作者：
Numata, Hideharu
中科院分区：
文献类型：
--
作者：
Takekata, Hiroki;Matsuura, Yu;Numata, Hideharu
昼夜节律的时钟机制长期以来一直存在争议，其分子基础完全未知。红树林蟋蟀Apteronemobius asahinai在其运动活动中同时表现出两种节律：产生活跃和不活跃阶段的周期节律以及调节周期活跃阶段活动强度的昼夜节律。利用RNAi技术，研究了昆虫生物钟的关键组成部分之一——时钟基因周期（per）在阿萨哈伊昼夜节律和周期节律中的作用。注射per双链RNA后，大多数蟋蟀没有表现出昼夜节律调节，但昼夜节律持续存在，与对照组没有显著差异。因此，per在功能上参与昼夜节律，但在昼夜节律中没有作用，或者作用不那么重要。我们认为，麻麻的昼夜节律受一种不同于昼夜节律的分子机制的昼夜节律钟控制。
The clock mechanism for circatidal rhythm has long been controversial, and its molecular basis is completely unknown. The mangrove cricket, Apteronemobius asahinai, shows two rhythms simultaneously in its locomotor activity: a circatidal rhythm producing active and inactive phases as well as a circadian rhythm modifying the activity intensity of circatidal active phases. The role of the clock gene period (per), one of the key components of the circadian clock in insects, was investigated in the circadian and circatidal rhythms of A. asahinai using RNAi. After injection of double-stranded RNA of per, most crickets did not show the circadian modulation of activity but the circatidal rhythm persisted without a significant difference in the period from controls. Thus, per is functionally involved in the circadian rhythm but plays no role, or a less important role, in the circatidal rhythm. We conclude that the circatidal rhythm in A. asahinai is controlled by a circatidal clock whose molecular mechanism is different from that of the circadian clock.