Identification of vitellogenin in the little skate (Raja erinacea).
Identification of vitellogenin in the little skate (Raja erinacea).
复制标题
小鳐鱼 (Raja erinacea) 中卵黄蛋白原的鉴定。
DOI：
10.1016/0305-0491(92)90393-6
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Callard,IP
中科院分区：
文献类型：
--
作者：
Perez,LE;Callard,IP
1. 采用MgCl2/EDTA选择性沉淀，deae -纤维素柱层析，从成熟雌性溜冰鞋中分离卵黄原蛋白。2. 在6.0% SDS-PAGE凝胶上鉴定出约205 kDa的单体。3. 此外，用硫酸铵分离卵黄蛋白可得到94和38 kDa的蛋白（推测的磷脂）和105、91和67 kDa的脂蛋白。4. 雌性和雄性冰鞋植入雌二醇后，体内磷酸盐掺入表明卵黄原蛋白被磷酸化。5. 总蛋白磷酸掺入量女性显著高于男性。6. 用雌二醇处理的男性溜冰鞋，磷酸盐掺入从植入后2天开始增加，在植入后约11天达到最大值。7. 对32p标记蛋白消失率的测定表明，正常女性滑冰血浆中的半衰期约为200小时。
1. Vitellogenin was isolated from mature female skates by selective precipitation with MgCl2/EDTA followed by chromatography on DEAE-cellulose columns. 2. A single monomer of approximately 205 kDa was identified on 6.0% SDS-PAGE gels. 3. In addition, isolation of yolk proteins with ammonium sulfate yielded proteins of 94 and 38 kDa (putative phosvitins) and putative lipovitellins of ca 105, 91 and 67 kDa. 4. In vivo phosphate incorporation in female and male skates implanted with estradiol indicated that vitellogenin was phosphorylated. 5. Total protein phosphate incorporation was significantly higher in females than male skates. 6. In male skates treated with estradiol, phosphate incorporation increased from 2 days after implantation to a maximum at approximately 11 days after implantation. 7. Determination of the rate of disappearance of 32P-labeled protein suggests a half-life of ca 200 hr in normal female skate plasma.