CHEMOTACTIC FACTORS FOR BOVINE NEUTROPHILS IN RELATION TO MASTITIS
CHEMOTACTIC FACTORS FOR BOVINE NEUTROPHILS IN RELATION TO MASTITIS
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DOI：
10.1016/0147-9571(86)90072-x
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发表时间：
1986-01-01
影响因子：
2
通讯作者：
CRAVEN, N
中科院分区：
文献类型：
--
作者：
CRAVEN, N
在琼脂糖作用下，研究了多种药物对牛血多形核中性粒细胞（PMN）的趋化作用。活性血清和白三烯B4具有显著的趋化活性，而各种细菌产物、甲酰基肽、酪蛋白和血小板活化因子未能吸引牛PMN。在体外培养的牛乳腺巨噬细胞中，当加入被抑制的、杀死的金黄色葡萄球菌、大肠杆菌或Zymosan或无菌大肠杆菌培养滤液或内毒素刺激时，释放出对同源PMN的趋化活性。未受刺激的巨噬细胞无明显活性。药理水平的各种抑制剂或花生四烯酸氧合对乳腺巨噬细胞产生PMN趋化剂无显著影响。
The chemotactic effet of a variety of agents for bovine blood polymorphonuclear neutrophils (PMN) was evaluated in vitro in assays of migration under agarose. Activated serum and leukotriene B4 showed significant chemotactic activity whereas various bacterial products, formyl peptides, casein and platelet activating factor failed to attract bovine PMN. In vitro cultures of bovine mammary gland macrophages released chemotactic activity for homologous PMN when stimulated by addition of opsonised, killed Staphylococcus aureus, Escherichia coli or Zymosan or sterile E. coli culture filtrates or endotoxin. No significant activity was produced by unstimulated macrophages. Pharmacological levels of various inhibitors or arachidonic acid oxygenation had no significant effect on the generation of PMN chemoattractants by mammary macrophages.