Molecular epidemiology of J-subgroup avian leukosis virus isolated from meat-type chickens in southern China between 2013 and 2014
Molecular epidemiology of J-subgroup avian leukosis virus isolated from meat-type chickens in southern China between 2013 and 2014
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2013-2014年华南地区肉用鸡J亚群禽白血病病毒分子流行病学分析
DOI：
10.1007/s00705-016-3003-8
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发表时间：
2016-11-01
影响因子：
2.7
通讯作者：
Xie, Qingmei
中科院分区：
文献类型：
--
作者：
Lin, Wencheng;Li, Xinjian;Xie, Qingmei
J亚群禽白血病病毒（ALV-J）引起多种与肿瘤形成和生育力下降相关的疾病，导致全球家禽业的重大经济损失。为了解我国南方地区肉鸡群中J群禽白血病病毒（ALV-J）的感染状况，对我国南方地区的J群禽白血病病毒（ALV-J）进行了分子流行病学调查。本研究调查了2013年至2014年从中国南方收集的265份临床样本中是否存在ALV-J，结果得到12株病毒分离株。系统进化分析表明，91.7%（11/12）的ALV-J分离株与中国蛋鸡分离株同源性较高，属于同一个亚群。其中一个ALV分离株（命名为GD 1411 -1）与鸡ALV-J分离株的亲缘关系较近，表明GD 1411 -1分离株可能是一个过渡株。12株ALV-J分离株均在p85和U3区发现了几处独特的核苷酸替换。这项研究提供了一些有趣的信息ALV-J分离株的分子特征。这些结果将有助于进一步了解ALV-J感染的致病机制。
Members of avian leukosis virus subgroup J (ALV-J) cause various diseases associated with tumor formation and decreased fertility, resulting in major economic losses in the poultry industry worldwide. To assess the status of ALV-J infection in meat-type chickens in southern China, the molecular epidemiology of ALV-J strains was investigated. A total of 265 clinical samples collected from southern China from 2013 to 2014 were investigated in this study for the presence of ALV-J, which resulted in 12 virus isolates. Phylogenetic analysis showed that 91.7 % (11/12) of the ALV-J isolates have possessed high homology to Chinese layer isolates and belong to one subgroup. One of the ALV isolates (designated GD1411-1) was relatively closely related to the ALV-J broiler isolates, indicating that the GD1411-1 isolate might be a transition strain. Several unique nucleotide substitutions ingp85and the U3 region were detected in all 12 ALV-J isolates. This study provides some interesting information on the molecular characterization of ALV-J isolates. These findings will be beneficial for understanding of the pathogenic mechanism of ALV-J infection.